RT Journal Article
T1 IC-Tagging methodology applied to the expression of viral glycoproteins and the difficult-to-express membrane-bound IGRP autoantigen
A1 Barreiro Piñeiro, Natalia
A1 Lostalé Seijo, Irene
A1 Varela Calviño, Rubén
A1 Benavente Martínez, Francisco Javier
A1 Martínez Costas, José Manuel
K1 Expression systems
K1 Microbiology techniques
K1 Type 1 diabetes
K1 Viral infection
AB We have previously developed a methodology to produce protein microspheres (MS) that can be loaded with proteins of interest in living cells through their C or N-terminal tagging with the so-called IC-Tag. The IC-Tagging method has many applications ranging from the production of immobilized enzymes for industrial use to the production of subunit vaccines due to its intrinsic adjuvancy. Here we show the adaptation of the IC-Tagging to work inside the endoplasmic reticulum and bacteria, allowing us to produce properly modified viral glycoproteins. Additionally, we were able to express the Islet-specific glucose-6-phosphatase catalytic subunit-related protein (IGRP), whose expression remained elusive to date possibly due to its toxicity when over-expressed. IGRP is an antigen of enormous pharmaceutical interest as it is specifically targeted during the autoimmune response taking place in both the Non-Obese Diabetic (NOD) mice and type 1 diabetes (T1D) patients leading to the destruction of insulin-producing beta cells
PB Springer Nature
YR 2018
FD 2018
LK http://hdl.handle.net/10347/18524
UL http://hdl.handle.net/10347/18524
LA eng
NO Barreiro-Piñeiro, N., Lostalé-Seijo, I., Varela-Calviño, R., Benavente, J., & Martínez-Costas, J. (2018). IC-Tagging methodology applied to the expression of viral glycoproteins and the difficult-to-express membrane-bound IGRP autoantigen. Scientific Reports, 8(1). doi: 10.1038/s41598-018-34488-3
NO This work was financed by the Spanish Ministerio de Economía y Competitividad, grant BFU2013-43513-R. Financial support from the Xunta de Galicia (Centro singular de investigación de Galicia accreditation 2016–2019) and the European Union (European Regional Development Fund - ERDF), is gratefully acknowledged. Irene Lostalé-Seijo was a recipient of a predoctoral FPU fellowship (Ministerio de Educación y Ciencia) and a Research Fellowship (Bolsa de Investigación; Deputación Provincial da Coruña)
DS Minerva
RD 24 abr 2026