RT Journal Article
T1 Polysaccharide nanoparticles can efficiently modulate the immune response against anHIV peptide antigen
A1 Gómez Dacoba, Tamara
A1 Omange, Robert W.
A1 Li, Hongzhao
A1 Crecente Campo, José
A1 Luo, Ma
A1 Alonso Fernández, María José
K1 HIV vaccine
K1 Peptide antigen
K1 Polysaccharide
K1 Nanovaccine
K1 Poly(I:C)
K1 Nanoparticle
K1 Antigen encapsulation
AB The development of an effective HIV vaccine continues to be a major health challenge since, so far, only the RV144 trial has demonstrated a modest clinical efficacy. Recently, the targeting of the 12 highly conserved protease cleavage sites (PCS1–12) has been presented as a strategy seeking to hamper the maturation and infectivity of HIV. To pursue this line of research, and because peptide antigens have low immunogenicity, we have included these peptides in engineered nanoparticles, aiming at overcoming this limitation. More specifically, we investigated whether the covalent attachment of a PCS peptide (PCS5) to polysaccharide-based nanoparticles, and their coadministration with polyinosinic:polycytidylic acid (poly(I:C)), improved the generated immune response. To this end, PCS5 was first conjugated to two different polysaccharides (chitosan and hyaluronic acid) through either a stable or a cleavable bond and then associated with an oppositely charged polymer (dextran sulfate and chitosan) and poly(I:C) to form the nanoparticles. Nanoparticles associating PCS5 by ionic interactions were used in this study as the control formulation. In vivo, all nanosystems elicited high anti-PCS5 antibodies. Nanoparticles containing PCS5 conjugated and poly(I:C) seemed to induce the strongest activation of antigen-presenting cells. Interestingly, T cell activation presented different kinetics depending on the prototype. These findings show that both the nanoparticle composition and the conjugation of the HIV peptide antigen may play an important role in the generation of humoral and cellular responses
PB American Chemical Society
SN 1936-0851
YR 2019
FD 2019
LK http://hdl.handle.net/10347/21322
UL http://hdl.handle.net/10347/21322
LA eng
NO Dacoba T.G., Omange R.W., Li H., Crecente-Campo J., Luo M., Alonso M.J., Polysaccharide nanoparticles can efficiently modulate the immune response against an HIV peptide antigen, ACS Nano, 13, 4947–4959 (2019) (DOI: 10.1021/acsnano.8b07662)
NO This work was supported by the National Institute of Allergy and Infectious Diseases of the National Institutes of Health (Award No. R01AI111805, Subaward No. 41795-02) and by the European Union’s Horizon 2020 research program (NanoPilot project, Grant Agreement No. 646142). T.G.D. acknowledges a predoctoral FPU grant from the Spanish Ministry of Education, Culture and Sports (Grant No. FPU14/05866)
DS Minerva
RD 28 abr 2026