RT Journal Article
T1 Galanin in an Agnathan: Precursor Identification and Localisation of Expression in the Brain of the Sea Lamprey Petromyzon marinus
A1 Sobrido Cameán, Daniel
A1 Yáñez Guerra, Luis Alfonso
A1 Lamanna, Francesco
A1 Conde Fernández, Candela
A1 Kaessmann, Henrik
A1 Elphick, Maurice R.
A1 Anadón Álvarez, Ramón
A1 Rodicio Rodicio, María Celina
A1 Barreiro Iglesias, Antón
K1 Lamprey
K1 Galanin
K1 Telencephalon
K1 Hypothalamus
K1 Striatum
K1 Neuropeptides
AB Galanin is a neuropeptide that is widely expressed in the mammalian brain, where it regulates many physiological processes, including feeding and nociception. Galanin has been characterized extensively in jawed vertebrates (gnathostomes), but little is known about the galanin system in the most ancient extant vertebrate class, the jawless vertebrates or agnathans. Here, we identified and cloned a cDNA encoding the sea lamprey (Petromyzon marinus) galanin precursor (PmGalP). Sequence analysis revealed that PmGalP gives rise to two neuropeptides that are similar to gnathostome galanins and galanin message-associated peptides. Using mRNA in situ hybridization, the distribution of PmGalP-expressing neurons was mapped in the brain of larval and adult sea lampreys. This revealed PmGalP-expressing neurons in the septum, preoptic region, striatum, hypothalamus, prethalamus, and displaced cells in lateral areas of the telencephalon and diencephalon. In adults, the laterally migrated PmGalP-expressing neurons are observed in an area that extends from the ventral pallium to the lateral hypothalamus and prethalamus. The striatal and laterally migrated PmGalP-expressing cells of the telencephalon were not observed in larvae. Comparison with studies on jawed vertebrates reveals that the presence of septal and hypothalamic galanin-expressing neuronal populations is highly conserved in vertebrates. However, compared to mammals, there is a more restricted pattern of expression of the galanin transcript in the brain of lampreys. This work provides important new information on the early evolution of the galanin system in vertebrates and provides a genetic and neuroanatomical basis for functional analyses of the galanin system in lampreys.
PB Frontiers Media
YR 2019
FD 2019
LK http://hdl.handle.net/10347/21163
UL http://hdl.handle.net/10347/21163
LA eng
NO Sobrido-Cameán D, Yáñez-Guerra LA, Lamanna F, Conde-Fernández C, Kaessmann H, Elphick MR, Anadón R, Rodicio MC and Barreiro-Iglesias A (2019) Galanin in an Agnathan: Precursor Identification and Localisation of Expression in the Brain of the Sea Lamprey Petromyzon marinus. Front. Neuroanat. 13:83. doi: 10.3389/fnana.2019.00083
NO Spanish Ministry of Economy and Competitiveness and the European Regional Development Fund 2007–2013 (Grant number: BFU-2017-87079-P). LY-G was supported by a Ph.D. studentship awarded by the Mexican Council of Science and Technology (CONACyT studentship no. 418612), and Queen Mary University of London
DS Minerva
RD 25 abr 2026