RT Journal Article
T1 CD26: A Negative Selection Marker for Human Treg Cells
A1 Salgado Castro, Francisco Javier
A1 Pérez-Díaz, Amparo
A1 Villanueva, Nora M.
A1 Lamas, Olaya
A1 Arias Crespo, María del Pilar
A1 Nogueira Álvarez, Montserrat
K1 CD26
K1 Regulatory T cells
K1 Effector T cells
K1 Flow cytometry
K1 Magnetic sorting
AB A major obstacle hampering the therapeutic application of regulatory T (Treg) cells is the lack of suitable extracellular markers, which complicates their identification/isolation. Treg cells are normally isolated via CD25 (IL-2Ra) targeting, but this protein is also expressed by activated CD41 effector T (Teff) lymphocytes. Other extracellular (positive or negative) Treg selection markers (e.g., HLA-DR, CD127) are also nonspecific. CD26 is an extracellular peptidase whose high expression has been traditionally used as an indicator of immune activation and effector functions in T cells. Now, we provide flow cytometry data showing high levels of CD26 within CD4+CD25- or CD4+FoxP3-/low effector T (Teff) lymphocytes, but negative or low levels (CD26-/low) in Treg cells selected according to the CD4+CD25high or the CD4+FoxP3high phenotype. Unlike the negative marker CD127 (IL-7Ra), which is down modulated in CD4+ Teff lymphocytes after TCR triggering, most of these cells upregulate CD26 and take a CD4+CD25+/highCD26+ phenotype upon activation. In contrast, there is only a slight upregulation within Treg cells (CD4+CD25highCD26-/low). Thus, differences in CD26 levels between Treg and Teff subsets are stable, and assessment of this marker, in combination with others like CD25, FoxP3, or CD127, may be useful during the quantitative evaluation or the isolation of Treg cells in samples containing activated Teff lymphocytes (e.g., from patients with autoimmune/inflammatory diseases).
PB John Wiley & Sons
SN 1552-4922
YR 2012
FD 2012-09-20
LK https://hdl.handle.net/10347/39466
UL https://hdl.handle.net/10347/39466
LA eng
NO Salgado, F.J., Pérez-Díaz, A., Villanueva, N.M., Lamas, O., Arias, P. and Nogueira, M. (2012), CD26: A negative selection marker for human Treg cells†. Cytometry, 81A: 843-855. https://doi.org/10.1002/cyto.a.22117
NO Xunta de Galicia. Grant Number: INCITE08PXIB200062PR
DS Minerva
RD 28 abr 2026