RT Journal Article
T1 The Effect of PEGylation on Multivalent Binding: A Surface Plasmon Resonance and Isothermal Titration Calorimetry Study with Structurally Diverse PEG‐Dendritic GATG Copolymers
A1 Fernández Villamarín, Marcos
A1 Sousa Hervés, Ana
A1 Correa, Juan
A1 Munoz, Eva Maria
A1 Taboada Antelo, Pablo
A1 Riguera Vega, Ricardo
A1 Fernández Megía, Eduardo
K1 Carbohydrate–lectin interactions
K1 Glycodendrimers
K1 Isothermal titration calorimetry
K1 Multivalency
K1 PEG-dendritic block copolymer
K1 Surface plasmon resonance
AB A general synthetic strategy to polyethylene glycol (PEG)‐dendritic block copolymers of the GATG (gallic acid–triethylene glycol) family is described from commercially available PEG of different molecular weights and architectures. Glycosylation of the resulting azide‐terminated copolymers with fucose by copper‐catalyzed azide–alkyne cycloaddition (CuAAC) afforded a toolbox to study the effect of PEG on the multivalent binding with the lectin UEA‐I by surface plasmon resonance (SPR, on surface) and isothermal titration calorimetry (ITC, in solution). Our results indicate that PEG reduces the affinity of glycodendrimers towards lectins by steric hindrance in a molecular‐weight‐dependent fashion. Great differences were observed as a function of the PEG architecture, with diblock PEG‐dendritic copolymers benefiting from a positive entropic contribution (PEG folding), not seen in the dendritic‐PEG‐dendritic systems. The self‐inflicted steric stabilization of the PEGylated copolymers onto lectin clusters reveals the necessity of additional competitive experiments to fully assess the antiadhesive properties of PEG in biological environments
PB Wiley
YR 2016
FD 2016-05-19
LK http://hdl.handle.net/10347/16964
UL http://hdl.handle.net/10347/16964
LA eng
NO Fernandez-Villamarin, M., Sousa-Herves, A., Correa, J., Munoz, E., Taboada, P., Riguera, R., & Fernandez-Megia, E. (2016). The Effect of PEGylation on Multivalent Binding: A Surface Plasmon Resonance and Isothermal Titration Calorimetry Study with Structurally Diverse PEG-Dendritic GATG Copolymers. Chemnanomat, 2(5), 437-446. doi: 10.1002/cnma.201600008
NO This is the peer-reviewed version of the following article: Fernandez-Villamarin, M., Sousa-Herves, A., Correa, J., Munoz, E., Taboada, P., Riguera, R., & Fernandez-Megia, E. (2016). The Effect of PEGylation on Multivalent Binding: A Surface Plasmon Resonance and Isothermal Titration Calorimetry Study with Structurally Diverse PEG-Dendritic GATG Copolymers. Chemnanomat, 2(5), 437-446, which has been published in final form at https://doi.org/10.1002/cnma.201600008. This article may be used for non-commercial purposes in accordance with Wiley-VCH Terms and Conditions for Self-Archiving
NO Spanish Government. Grant Numbers: CTQ2015-69021-R, CTQ2012-34790, CTQ2014-61470-EXPXunta de Galicia. Grant Number: GRC2014/040
DS Minerva
RD 4 may 2026