RT Journal Article
T1 mRNA-activated matrices encoding transcription factors as primers of cell differentiation in tissue engineering
A1 Martínez Ledo, Adriana
A1 Senra, Ana
A1 Rilo Álvarez, Héctor
A1 Borrajo Alonso, Erea
A1 Vidal Figueroa, Anxo
A1 Alonso Fernández, María José
A1 García Fuentes, Marcos
K1 GAMs
K1 3D transfection
K1 mRNA
K1 Transcription factors
K1 Lineage priming
K1 Tissue engineering
AB Gene-activated matrices (GAMs) encoding pivotal transcription factors (TFs) represent a powerful tool to direct stem cell specification for tissue engineering applications. However, current TF-based GAMs activated with pDNA, are challenged by their low transfection efficiency and delayed transgene expression. Here, we report a GAM technology activated with mRNAs encoding TFs SOX9 (cartilage) and MYOD (muscle). We find that these mRNA-GAMs induce a higher and faster TF expression compared to pDNA-GAMs, especially in the case of RNase resistant mRNA sequences. This potent TF expression was translated into a high synthesis of cartilage- and muscle-specific markers, and ultimately, into successful tissue specification in vitro. Additionally, we show that the expression of tissue-specific markers can be further modulated by altering the properties of the mRNA-GAM environment. These results highlight the value of this GAM technology for priming cell lineage specification, a key centerpiece for future tissue engineering devices.
PB Elsevier
SN 0142-9612
YR 2020
FD 2020
LK http://hdl.handle.net/10347/23240
UL http://hdl.handle.net/10347/23240
LA eng
NO Ledo, A. M., Senra, A., Rilo-Alvarez, H., Borrajo, E., Vidal, A., Alonso, M. J., & Garcia-Fuentes, M. (2020). mRNA-activated matrices encoding transcription factors as primers of cell differentiation in tissue engineering. Biomaterials, 247, 120016-120016.
NO This work has been funded by Ministerio de Economía y Competitividad (MINECO-RETOS, Grant MAT2017-84361-R, Feder Funds), Fundación BBVA 2014-PO0110 and Xunta de Galicia (Grupos de Referencia Competitiva, Feder Funds; Convenio para fomentar a actividade investigadora do persoal investigador finalista nas convocatorias de axudas do ERC no marco da H2020). AML was a recipient of a FPU grant from Ministerio de Economía y Competitividad (FPU12/05528)
DS Minerva
RD 25 abr 2026