RT Journal Article
T1 Simplified engineering design towards a competitive lipid-rich effluents valorization
A1 Argiz Montes, Lucía
A1 Val del Río, Ángeles
A1 Correa Galeote, David
A1 Rodelas González, Belén
A1 Mosquera Corral, Anuska
K1 Bioprocess
K1 Mixed microbial culture
K1 Triacylglyceride
K1 Polyhydroxyalkanoate
K1 Waste lipids
AB Medium- and long-chain fatty acids and glycerol contained in the oily fraction of many food-industry effluents are excellent candidates to produce biobased high-value triacylglycerides (TAGs) and polyhydroxyalkanoates (PHAs). The typical process configuration for TAGs recovery from lipid-rich streams always includes two steps (culture enrichment plus storage compounds accumulation) whereas, for PHAs production, an additional pretreatment of the substrate for the obtainment of soluble volatile fatty acids (VFAs) is required. To simplify the process, substrate hydrolysis, culture enrichment, and accumulation (TAG and PHA storage) were coupled here in a single sequencing batch reactor (SBR) operated under the double growth limitation strategy (DGL) and fed in pulses with industrial waste fish oil during the whole feast phase. When the SBR was operated in 12 h cycles, it was reached up to 51 wt % biopolymers after only 6 h of feast (TAG:PHA ratio of 50:51; 0.423 CmmolBIOP/CmmolS). Daily storage compound production was observed to be over 25% higher than the reached when enrichment and accumulation stages were carried in separate operational units. Increasing the feast phase length from 6 to 12 h (18 h cycle) negatively affected the DGL strategy performance and hence system storage capacity, which was recovered after also extending the famine phase in the same proportion (24 h cycle). Besides, the carbon influx during the feast phase was identified as a key operational parameter controlling storage compounds production and, together with the C/N ratio, culture selection. The different cycle configurations tested clearly modulated the total fungal abundances without no significant differences in the size of the bacterial populations. Several PHA and TAG producers were found in the mixed culture although the PHA and TAG productions were poorly associated with the increased relative abundances (RAs) of specific operational taxonomic units (OTUs)
PB Elsevier
YR 2022
FD 2022
LK http://hdl.handle.net/10347/29100
UL http://hdl.handle.net/10347/29100
LA eng
NO Journal of Environmental Management 317 (2022) 115433
NO This research was supported by the Spanish Government (AEI, Spain) through the TREASURE project [CTQ 2017-83225-C2-1-R]. Lucía Argiz is a Xunta de Galicia fellow [ED 484A-2019/083] (GAIN, Galicia, Spain), grant is co-funded by the operative program FSE Galicia 2014–2020. The authors belong to the Galician Competitive Research Group GRC ED431C 2017/29. All these programs are co-funded by the FEDER (EU)
DS Minerva
RD 30 abr 2026