RT Journal Article
T1 IL-36α: a novel cytokine involved in the catabolic and inflammatory response in chondrocytes
A1 Conde, Javier
A1 Scotece, Morena
A1 Abella, Vanessa
A1 Lois, Ana
A1 López, Verónica
A1 García-Caballero Parada, Tomás
A1 Pino, Jesús
A1 Gómez-Reino Carnota, Juan Jesús
A1 Gómez, Rodolfo
A1 Lago Paz, María Francisca
A1 Gualillo, Oreste
AB Recent studies confer to IL-36α pro-inflammatory properties. However, little is known about the expression and function of IL-36α in cartilage. This study sought to analyze the expression of IL-36α in healthy and OA cartilage. Next, we determined the effects of recombinant IL-36α on catabolism and inflammation in chondrocytes. For completeness, part of the signaling pathway elicited by IL-36α was also explored. IL-36α expression was evaluated by immunohistochemistry and RT-qPCR. Expression of MMP-13, NOS2 and COX-2 was also determined in OA articular chondrocytes treated with recombinant IL-36α. IκB-α and P-p38 was explored by western blot. We observed a low constitutive expression of IL-36α in healthy human chondrocytes. However, OA chondrocytes likely expressed more IL-36α than healthy chondrocytes. In addition, immune cells infiltrated into the joint and PBMCs express higher levels of IL-36α in comparison to chondrocytes. OA chondrocytes, treated with IL-36α, showed significant increase in the expression of MMP-13, NOS2 and COX-2. Finally, IL-36α stimulated cells showed NFκB and p38 MAPK activated pathways. IL-36α acts as a pro-inflammatory cytokine at cartilage level, by increasing the expression of markers of inflammation and cartilage catabolism. Like other members of IL-1 family, IL-36α acts through the activation of NFκB and p38 MAPK pathway.
PB Nature Publishing Group
SN 2045-2322
YR 2015
FD 2015
LK http://hdl.handle.net/10347/22193
UL http://hdl.handle.net/10347/22193
LA eng
NO Conde, J., Scotece, M., Abella, V. et al. IL-36α: a novel cytokine involved in the catabolic and inflammatory response in chondrocytes. Sci Rep 5, 16674 (2015). https://doi.org/10.1038/srep16674
NO Oreste Gualillo and Francisca Lago are Staff Personnel of Xunta de Galicia (SERGAS) through a research-staff stabilization contract (ISCIII/SERGAS). OG is a member of RETICS Programme, RD12/0009/0008 (RIER: Red de Investigación en Inflamación y Enfermedades Reumáticas) via FEDER and Instituto de Salud Carlos III (ISCIII). The OG work was funded by Instituto de Salud Carlos III and FEDER (grants PI14/00016 and PIE13/00024). Vanessa Abella is a recipient of a predoctoral fellowship from ESF (European Social Fund) grant from Xunta de Galicia through a contract signed with University of Coruña. Morena Scotece is recipient of the “FPU” Program of the Spanish Ministry of Education. Javier Conde is a recipient of a fellowship from the Foundation IDIS-Ramón Dominguez
DS Minerva
RD 24 abr 2026