RT Journal Article
T1 NeuroTorp, a Lateral Flow Test Based on Toxin-Receptor affinity for in-situ early detection of cyclic imine toxins
A1 Noirmain, Fanny
A1 Dano, Julie
A1 Hue, Nathalie
A1 González Jartín, Jesús María
A1 Botana López, Luis Miguel
A1 Servent, Denis
A1 Simon, Stéphanie
A1 Aráoz, Rómulo
K1 Lateral flow test
K1 Nicotinic acetylcholine receptors
K1 Receptor ligand binding assay
K1 Cyclic imine toxins
K1 Phycotoxins detection
K1 Harmful algal blooms
AB The emergent cyclic imine toxins produced by marine dinoflagellates are potent antagonists of nicotinic acetylcholine receptors. Shellfish accumulate cyclic imine toxins following filter-feeding on toxic dinoflagellates vectoring them to humans. Herein is presented a lateral flow test for the detection of cyclic imine toxins based on three new concepts for test strips: i) the immobilization of lipoprotein vesicles in the test-line, ii) the high affinity of neurotoxins for their receptor targets and iii) the use of high porosity glass fiber filter membranes as support for the fabrication of the lateral flow test NeuroTorp (WO2017108115). Purified electrocyte membrane vesicles from Torpedo marmorata were used as a source of receptor and were immobilized in the test-line. Biotin-α-bungarotoxin was used as toxin tracer for the NeuroTorp LFT given its high affinity for nicotinic acetylcholine receptors while neutravidin nanogold particle conjugates enable its visual detection. Herein is reported for the first time the use of GF/C glass fiber membranes as the stationary phase for a lateral flow test. The GF/C filter ensures both: the immobilization of a complex lipoprotein in the test-line and the capillary migration of the mobile phase. Scanning electron microscopy studies shed light into the mechanism by which Torpedo-electrocyte membranes vesicles are immobilized in the GF/C glass microfiber. The electrocyte membrane vesicles anchor in neighboring microfibers randomly disposed in the same plane of the GF/C filter forming stable microfilm structures ensuring the functionality of nicotinic acetylcholine receptors. NeuroTorp is a ready-to-use low-cost early warning device for rapid detection of cyclic imine toxins in shellfish by end-users
PB Elsevier
YR 2022
FD 2022
LK http://hdl.handle.net/10347/33736
UL http://hdl.handle.net/10347/33736
LA eng
NO Noirmain F, Dano J, Hue N, Gonzalez-Jartin JM, Botana LM, Servant D, Simon S and Aráoz R. NeuroTorp, a lateral flow test based on toxin-receptor affinity for in-situ early detection of cyclic imine toxins. Anal. chim. Acta. 2022 aug.; 1221: 339941
NO The authors acknowledge the funding support from the LABEX LERMIT (DETECTNEUROTOX project, CDE 2017–001173 – RD 91 to RA), the NRBC-E Program (MULTITOX project, Fiche N° H35 to RA) and the INTERREG Atlantic Area (ALERTOX-NET EAPA_317/2016 project to LMB and DS). The GDR PHYCOTOX is acknowledged for financial support to RA. We acknowledge Michaël Trichet from the IBPS electron microscopy core facility (https://www.ibps.sorbonne-universite.fr/en/core-facilities/imaging/electron-microscopy-facility) and the support from «Région Île-de-France», from Sorbonne-Université and from CNRS.
DS Minerva
RD 28 abr 2026