RT Journal Article
T1 Residues of anilinopyrimidine fungicides and suspected metabolites in wine samples
A1 Castro Varela, Gabriela
A1 Pérez Mayán, Leticia
A1 Carpinteiro Botana, María Inmaculada
A1 Ramil Criado, María
A1 Cela Torrijos, Rafael
A1 Rodríguez Pereiro, Isaac
K1 Wine
K1 Pyrimethanil
K1 Cyprodinil
K1 Metabolites
K1 Liquid chromatography-mass spectrometry
AB The coexistence of the anilinopyrimidine fungicides pyrimethanil (PYR) and cyprodinil (CYP), and suspected metabolites in wine samples was investigated by liquid chromatography (LC) with tandem mass spectrometry (MS/MS), based on triple quadrupole (QqQ) and quadrupole time-of-flight (QTOF) MS instruments. For the first time, quantitative data obtained after solid-phase extraction (SPE) of wine samples have demonstrated the systematic presence of 4-hydroxyanilino derivatives of PYR and CYP in wines containing residues of parent fungicides, at concentrations from 0.2 to 58 ng mL−1. Higher concentration ratios (hydroxylated derivative/active fungicide) were measured in red than in white wines, particularly in case of PYR. On average, the concentrations of PYR-4OH were twice those measured for PYR in red wines. A targeted search of hydroxyl derivatives in wine extracts by LC-QTOF-MS showed the existence of additional hydroxylation positions in the pyrimidine ring and/or in the alkyl substituents bond to this cycle in the structure of both anti-botrytis fungicides. Moreover, free and glycosylated forms of the hydroxylated metabolites for both fungicides coexist in wine samples. In case of CYP, it is proved that hydroxylated and glycosylated metabolites are already present in grapes before vinification
PB Elsevier
SN 0021-9673
YR 2020
FD 2020
LK http://hdl.handle.net/10347/22365
UL http://hdl.handle.net/10347/22365
LA eng
NO G. Castro, L. Pérez-Mayán and I. Carpinteiro et al., Residues of anilinopyrimidine fungicides and suspected metabolites in wine samples, Journal of Chromatography A, https://doi.org/10.1016/j.chroma.2020.461104
NO L.P.M acknowledges a FPU grant to the Spanish Ministry of Science. The study was supported by Xunta de Galicia, Spanish Government and EU funds through grants GRC-ED431C 2017/36, PGC2018-094613-B-I00 and the Interreg SUDOE VINOVERT project
DS Minerva
RD 24 abr 2026