RT Journal Article
T1 Faster monitoring of the invasive alien species (IAS) Dreissena polymorpha in river basins through isothermal amplification
A1 Carvalho, Joana
A1 Garrido Maestu, Alejandro
A1 Azinheiro, Sarah
A1 Fuciños, Pablo
A1 Barros Velázquez, Jorge
A1 Miguel, Ramón José de
A1 Gros, Verónica
A1 Prado Rodríguez, Marta
K1 Analytical biochemistry
K1 Ecology
K1 Environmental sciences
K1 Freshwater ecology
K1 Invasive species
K1 Natural hazards
AB Zebra mussel (Dreissena polymorpha) is considered as one of the 100 most harmful IAS in the world. Traditional detection methods have limitations, and PCR based environmental DNA detection has provided interesting results for early warning. However, in the last years, the development of isothermal amplification methods has received increasing attention. Among them, loop-mediated isothermal amplification (LAMP) has several advantages, including its higher tolerance to the presence of inhibitors and the possibility of naked-eye detection, which enables and simplifies its potential use in decentralized settings. In the current study, a real-time LAMP (qLAMP) method for the detection of Dreissena polymorpha was developed and tested with samples from the Guadalquivir River basin, together with two real-time PCR (qPCR) methods using different detection chemistries, targeting a specific region of the mitochondrial gene cytochrome C oxidase subunit I. All three developed approaches were evaluated regarding specificity, sensitivity and time required for detection. Regarding sensitivity, both qPCR approaches were more sensitive than qLAMP by one order of magnitude, however the qLAMP method proved to be as specific and much faster being performed in just 9 min versus 23 and 29 min for the qPCR methods based on hydrolysis probe and intercalating dye respectively
YR 2021
FD 2021
LK http://hdl.handle.net/10347/26804
UL http://hdl.handle.net/10347/26804
LA eng
NO Sci Rep 2021, 11: 10175. https://doi.org/10.1038/s41598-021-89574-w
NO This work was supported by a partnership agreement project between the Confederación Hidrográfica del Guadalquivir and INL for the development of a system for early detection of zebra mussels through analysis of environmental DNA, and by project Nanotechnology Based Functional Solutions (NORTE-01-0145-FEDER-000019), supported by Norte Portugal Regional Operational Programme (NORTE2020), under the PORTUGAL 2020 Partnership Agreement, through the European Regional Development Fund. S. A. acknowledges the Portuguese funding institution FCT – Fundação para Ciência e Tecnologia for Ph.D. scholarship SFRH/BD/140396/2018
DS Minerva
RD 24 abr 2026