RT Journal Article
T1 In Situ Antibacterial Activity of Essential Oils with and without Alcohol on Oral Biofilm: A Randomized Clinical Trial
A1 Quintas González, Víctor
A1 Prada López, Isabel
A1 Carreira Nouche, María José
A1 Suárez Quintanilla, David
A1 Balsa Castro, Carlos
A1 Tomás Carmona, Inmaculada
K1 Anti-infective agents
K1 Local
K1 Biofilm
K1 Dental plaque
K1 Essential oils
K1 Microscopy
K1 Fluorescence
AB Currently, there is little evidence on the in situ antibacterial activity of essential oils (EO) without alcohol. This study aimed to evaluate in situ the substantivity and antiplaque effect on the plaque-like biofilm (PL-biofilm) of two solutions, a traditional formulation that contains EO with alcohol (T-EO) and an alcohol-free formulation of EO (Af-EO). Eighteen healthy adults performed a single mouthwash of: T-EO, Af-EO, and sterile water (WATER) after wearing an individualized disk-holding splint for 2 days. The bacterial viability (BV) and thickness of the PL-biofilm were quantified at baseline, 30 s, and 1, 3, 5, and 7 h post-rinsing (Test 1). Subsequently, each volunteer wore the splint for 4 days, applying two daily mouthwashes of: T-EO, Af-EO, and WATER. The BV, thickness, and covering grade (CG) of the PL-biofilm were quantified (Test 2). Samples were analyzed by confocal laser scanning microscopy after staining with the LIVE/DEAD® BacLight™ solution. To conduct the computations of the BV automatically, a Matlab toolbox called Dentius Biofilm was developed. In test 1, both EO antiseptics had a similar antibacterial effect, reducing BV after a single rinse compared to the WATER, and keeping it below baseline levels up to 7 h post-rinse (P < 0.001). The mean thickness of the PL-biofilm after rinsing was not affected by any of the EO formulations and ranged from 18.58 to 20.19 μm. After 4 days, the T-EO and Af-EO solutions were significantly more effective than the WATER, reducing the BV, thickness, and CG of the PL-biofilm (P < 0.001). Although, both EO antiseptics presented a similar bactericidal activity, the Af-EO rinses led to more significant reductions in the thickness and CG of the PL-biofilm than the T-EO rinses (thickness = 7.90 vs. 9.92 μm, P = 0.012; CG = 33.36 vs. 46.61%, P = 0.001). In conclusion, both essential oils antiseptics had very high immediate antibacterial activity and substantivity in situ on the 2-day PL-biofilm after a single mouthwash. In the 4-day PL-biofilm, both essential oils formulations demonstrated a very good antiplaque effect in situ, although the alcohol-free formula performed better at reducing the biofilm thickness and covering grade
PB Frontiers Media
YR 2017
FD 2017
LK http://hdl.handle.net/10347/17713
UL http://hdl.handle.net/10347/17713
LA eng
NO Quintas V, Prada-López I, Carreira MJ, Suárez-Quintanilla D, Balsa-Castro C and Tomás I (2017) In Situ Antibacterial Activity of Essential Oils with and without Alcohol on Oral Biofilm: A Randomized Clinical Trial. Front. Microbiol. 8:2162. doi: 10.3389/fmicb.2017.02162
NO This investigation was supported by the 2014-CE2010 project financed by Johnson & Johnson Company, Madrid, Spain. Specifically, the development of the Matlab toolbox called Dentius Biofilm received financial support from the Consellería de Cultura, Educación e Ordenación Universitaria (accreditation 2016-2019, ED431G/08) and the European Regional Development Fund (ERDF)
DS Minerva
RD 24 abr 2026