RT Journal Article
T1 Directed self-assembly of trimeric DNA-bindingchiral miniprotein helicates
A1 Gómez González, Jacobo
A1 Peña Gil, Diego
A1 Barka, Ghofrane
A1 Sciortino, Giuseppe
A1 Maréchal, Jean-Didier
A1 Vázquez López, Miguel
A1 Vázquez Sentís, Marco Eugenio
K1 Metallopeptide
K1 Self-assembly water
K1 DNA recognition
K1 Enantioselectivity
K1 Peptide motifs
K1 Coordination chemistry
AB We propose that peptides are highly versatile platforms for the precise design of supramolecular metal architectures, and particularly, for the controlled assembly of helicates. In this context, we show that the bacteriophage T4 Fibritin foldon (T4Ff) can been engineered on its N-terminus with metal-chelating 2,2′-bipyridine units that stereoselectively assemble in the presence of Fe(II) into parallel, three-stranded peptide helicates with preferred helical orientation. Modeling studies support the proposed self-assembly and the stability of the final helicate. Furthermore, we show that these designed mini-metalloproteins selectively recognize three-way DNA junctions over double-stranded DNA
PB Frontiers Media
YR 2018
FD 2018
LK http://hdl.handle.net/10347/22701
UL http://hdl.handle.net/10347/22701
LA eng
NO Gómez-González J, Peña DG, Barka G, Sciortino G, Maréchal J-D, Vázquez López M and Vázquez ME (2018) Directed Self-Assembly of Trimeric DNA-Bindingchiral Miniprotein Helicates. Front. Chem. 6:520. doi: 10.3389/fchem.2018.00520
NO Financial support from the Spanish grants CTQ2015-70698-R, CTQ2017-87889-P, the Xunta de Galicia (Centro singular de investigación de Galicia accreditation 2016–2019, ED431G/09) and the European Union (European Regional Development Fund - ERDF), is gratefully acknowledged. JG-G, thanks the Spanish MINECO for his FPI fellowship, GB thanks the ERC for her EU METALIC-II 2013-2442/001-001-EMA2 mobility scheme fellowship, and GS. thanks the Universitat Autònoma de Barcelona for its support to his PhD. J-DM and GS are thankful for the support given by the Generalitat de Catalunya 2017SGR1323. Support of COST Action CM1306 is kindly acknowledged. MEV, also wish to acknowledge the generous support by the Fundación Asociación Española Contra el Cáncer AECC (IDEAS197VAZQ grant)
DS Minerva
RD 28 abr 2026