RT Journal Article
T1 In Search of Antioxidant Peptides from Porcine Liver Hydrolysates Using Analytical and Peptidomic Approach
A1 López-Pedrouso, María
A1 Lorenzo, José M.
A1 Borrajo, Paula
A1 Franco Ruiz, Daniel
K1 Amino acid profile
K1 Alcalase
K1 Bromelain
K1 Flavourzyme
K1 Papain
K1 Pork by-products
AB The search for antioxidant peptides as health-promoting agents is of great scientific interest for their biotechnological applications. Thus, the main goal of this study was to identify antioxidant peptides from pork liver using alcalase, bromelain, flavourzyme, and papain enzymes. All liver hydrolysates proved to be of adequate quality regarding the ratio EAA/NEAA, particularly flavourzyme hydrolysates. The peptidomic profiles were significantly different for each enzyme and their characterizations were performed, resulting in forty-four differentially abundant peptides among the four treatments. Porcine liver hydrolysates from alcalase and bromelain are demonstrated to have the most antioxidant capacity. On the other hand, hydrophobic amino acid residues (serine, threonine, histidine and aspartic acid) might be reducing the hydrolysates antioxidant capacity. Seventeen peptides from collagen, albumin, globin domain-containing protein, cytochrome , fructose-bisphosphate aldolase, dihydropyrimidinase, argininosuccinate synthase, and ATP synthase seem to be antioxidant. Further studies are necessary to isolate these peptides and test them in in vivo experiments
PB MDPI
YR 2021
FD 2021-12-24
LK https://hdl.handle.net/10347/39080
UL https://hdl.handle.net/10347/39080
LA eng
NO López-Pedrouso, M., Lorenzo, J. M., Borrajo, P., & Franco, D. (2021). In search of antioxidant peptides from porcine liver hydrolysates using analytical and peptidomic approach. Antioxidants, 11(1), 27.
DS Minerva
RD 28 abr 2026