RT Journal Article T1 Presence of Porcine Circovirus Type 2 in the Environment of Farm Facilities without Pigs in Long Term-Vaccinated Farrow-to-Wean Farms A1 López-Lorenzo, Gonzalo A1 Prieto Lago, Alberto A1 López Novo, Cynthia A1 Díaz Fernández, Pablo A1 Remesar Alonso, Susana A1 Morrondo Pelayo, María Patrocinio A1 Fernández, Gonzalo A1 Díaz Cao, José Manuel K1 Biosecurity K1 Environmental sampling K1 Porcine circovirus type 2 (PCV2) K1 Real-time PCR K1 Swine AB Vaccination against Porcine Circovirus Type 2 (PCV2) even over several years has proven as an insufficient measure to eradicate the infection from farms, possibly due to not producing sterilizing immunity. Viral persistence in the farm environment has been proposed as a possible cause of reinfection, and for that reason, the main objective of this study was to identify potential critical points where PCV2 could persist in farrow-to-wean farms which had been vaccinating piglets for years. Surface samples were collected from different farm facilities with and without animals and analyzed by qPCR to detect and quantify the viral load. Most of the samples taken in animal housing facilities tested negative (96.6%); however, PCV2 was more frequently detected in samples from the offices (37.5%), the farm staff (25%) and the perimeter (21%). These results indicate that PCV2 contamination is frequent in facilities despite the long-term use of vaccination programs. Therefore, PCV2 control programs should include more exhaustive cleaning and disinfection protocols in non-animal facilities, as well as the implementation of specific biosecurity measures in these areas to minimize the risk of PCV2 introduction from external sources PB MDPI YR 2022 FD 2022 LK http://hdl.handle.net/10347/32180 UL http://hdl.handle.net/10347/32180 LA eng NO López-Lorenzo, G.; Prieto, A.; López-Novo, C.; Díaz, P.; Remesar, S.; Morrondo, P.; Fernández, G.; Díaz-Cao, J.M. Presence of Porcine Circovirus Type 2 in the Environment of Farm Facilities without Pigs in Long Term-Vaccinated Farrow-to-Wean Farms. Animals 2022, 12, 3515. https://doi.org/10.3390/ani12243515 NO This research has been funded by the European PCV2-Award sponsored by Boehringer Ingelheim Animal Health, Germany. We would like to thank COREN and the farmers for their disposition to develop the present study and Martín Rodríguez Villar for his collaboration in the samplings and his technical assistance. DS Minerva RD 24 abr 2026