RT Journal Article
T1 Are Environmental Levels of Nonsteroidal Anti-Inflammatory Drugs a Reason for Concern? Chronic Life-Cycle Effects of Naproxen in Zebrafish
A1 Barros, Susana
A1 Montes Goyanes, Rosa
A1 Rodil Rodríguez, María del Rosario
A1 Quintana Álvarez, José Benito
A1 Neuparth, Teresa
K1 Contaminants of emerging concern
K1 Nonsteroidal anti-inflammatory drugs
K1 Danio rerio
K1 Histopathology
K1 RNA-seq
K1 Endocrine disruption
K1 Energy metabolism
K1 Epigenome
K1 Epitranscriptome
AB The nonsteroidal anti-inflammatory drug naproxen (NPX) is among the most consumed pharmaceuticals worldwide, being detected in surface waters within the ng to μg/L range. Considering the limited chronic ecotoxicity data available for NPX in aquatic ecosystems, the present study aimed at evaluating its impact in the model organism Danio rerio, following a full life-cycle exposure to environmentally relevant concentrations (0.1 to 5.0 μg/L). An integration of apical endpoints, i.e., survival, growth, and reproduction, with gonad histopathology and gene transcription (RNA-seq) was performed to provide additional insights into the mode of action (MoA) of NPX. NPX decreased zebrafish growth and reproduction and led to histopathological alterations in gonads at concentrations as low as 0.1 μg/L. At the molecular level, 0.7 μg/L of NPX led to a disruption in gonads transcription of genes involved in several biological processes associated with reproduction, mainly involving steroid hormone biosynthesis and epigenetic/epitranscriptomic machineries. Collectively, these results show that environmentally realistic concentrations of NPX affect zebrafish reproduction and associated signaling pathways, indicating that current hazard and risk assessment data for NPX underestimate the environmental risk of this pharmaceutical.
PB ACS
YR 2024
FD 2024-10-24
LK https://hdl.handle.net/10347/37917
UL https://hdl.handle.net/10347/37917
LA eng
NO S. Barros et al. Environ. Sci. Technol. 58 (2024) 19627-19638
NO This document is the unedited Author’s version of a Submitted Work that was subsequently accepted for publication in Environmental Science & Technology (ES&T), copyright © 2024 American Chemical Society, after peer review. To access the final edited and published work see http://pubs.acs.org/page/copyright/permissions.html.
NO This work was developed under the projects: (1) TRANSEPIC – Exploring Transgenerational Epigenetic Inheritance: New Methods and Strategies to Improve Environmental Hazard and Risk Assessment of Key Contaminants of Emerging Concern (CECs) [reference: 2022.02922.PTDC, doi: 10.54499/2022.02922.PTDC], financed by the Portuguese Foundation for Science and Technology (FCT), and (2) BLUEWWATER project – “Controlo, tratamento e redução de microplásticos e poluentes emergentes nas águas residuais urbanas e no ambiente costeiro transfronteiriço” reference: 0048_BLUE_WWATER_1_E, cofinanced by the European Union through the Interreg VI-A Program Spain-Portugal (POCTEP) 2021–2027. National Funds for CIIMAR (UIDB/04423/2020; UIDP/04423/2020), CITAB (UIDB/04033/2020, DOI: 10.54499/UIDB/04033/2020) and Inov4Agro (LA/P/0126/2020, DOI: 10.54499/LA/P/0126/2020), were also obtained through FCT. T.N. acknowledges FCT Individual Call to Scientific Employment Stimulus 2022 (2022.02925.CEECIND/CP1728/CT0004, doi: 10.54499/2022.02925.CEECIND/CP1728/CT0004); S.B., M.P., H.M., M.R., and N.A. acknowledge FCT for their Ph.D. grants PD/BD/143090/2018; SFRH/BD/147834/2019; SFRH/BD/139762/2018; 2022.12763.BD and DFA/BD/6218/2020, respectively. R.M., R.R., and J.B.Q. also want to acknowledge funding provided by Xunta de Galicia (ED431C 2021/06) and the Spanish Agencia Estatal de Investigación – MCIN/AEI/10.13039/501100011033 (ref PID2020–117686RB-C32).
DS Minerva
RD 20 may 2026