RT Journal Article
T1 Quantitative flow cytometry to measure viral production using infectious pancreatic necrosis virus as a model: a preliminary study
A1 Vázquez, Diego
A1 López Vázquez, Carmen
A1 Olveira Hermida, José Gabriel
A1 Bandín Matos, Isabel
A1 Pereira Dopazo, Carlos
K1 qFCM
K1 IPNV
K1 Viral replication
AB In recent decades, flow cytometry (FCM) has become an important tool in virology, due to its applications in viral replication and viral-cell interactions, as well as its capacity to quantify proteins (qFCM). In the present study, we have designed and evaluated a qFCM procedure for the in vitro analysis and quantification of fish viral proteins, using the infectious pancreatic necrosis virus (IPNV) as a model. We have also tested its use for viral titration and adapted the MARIS (method for analysing RNA following intracellular sorting) method for simultaneous quantification of viral RNA expression in infected cells. The procedure has proved to be repeatable and reproducible to an acceptable level, although to ensure reproducibility, the repetition of standard curves is inevitable. Regarding its use for viral quantification, a direct relationship (by a second-degree polynomial regression) between viral titres and Molecules of Equivalent Soluble Fluorochrome (MESF) was observed. Finally, the results support the use of this technology, not only for virus quantification, but also to study viral replication from a quantitative approach
PB MDPI
YR 2018
FD 2018
LK http://hdl.handle.net/10347/22625
UL http://hdl.handle.net/10347/22625
LA eng
NO Vázquez, D.; López-Vázquez, C.; Olveira, J.G.; Bandín, I.; Dopazo, C.P. Quantitative Flow Cytometry to Measure Viral Production Using Infectious Pancreatic Necrosis Virus as a Model: A Preliminary Study. Appl. Sci. 2018, 8, 1734
DS Minerva
RD 24 abr 2026