RT Journal Article T1 Effects of Concentrate Source (Cottonseed vs. Barley) on Milk Performance and Fatty Acids Profile of Holstein-Friesian Dairy Cows A1 Roca Fernández, Ana Isabel A1 González-Rodríguez, A. A1 Vázquez-Yáñez, O. P. A1 Fernández-Casado, J. A. K1 Cottonseed K1 Linoleic acid K1 Lipid feed supplements K1 Milk fat content K1 Total mixed ration AB Milk performance (protein and fat content) and milk fatty acids (FA) profile of dairy cows under supplementation with two sources of concentrate, oilseeds (C, cottonseed) at two levels, low (5kg DM/cow/day) and high (7 kg DM/cow/day), and cereal grains (B, barley) at high rate (7 kg DM/cow/day), were studied in three herds (n=36) of spring calving Holstein-Friesian dairy cows (200 days in milk). Animals were randomly assigned to one of three indoors (n=12) silage feeding regimes (C5, C7 and B7), using a TMR (total mixed ration) basal diet, containing 70-80% silage (grass: maize, 36: 64) and 30-20% concentrate. Daily milk yield (MY) was higher (P<0.001) at high level of supplementation (B7, 18.1 and C7, 17.9 kg/cow/day, respectively) compared to low level (C5, 15.7 kg/cow/day), and dairy cows at the highest level of concentrate showed the highest (P<0.05) body weight (B7, 605 and C7, 598, respectively). Milk protein content was lower (P<0.05) in the high level of cottonseed (C7, 30.7 g/kg DM) than in the barley treatment (B7, 32.7 g/kg DM). There were no differences among treatments in milk fat and milk urea content. Weekly milk FA profile of cow milk was determined using gas chromatography-mass spectrometry, during seventy days in autumn, and no differences were found among treatments in short, medium and long chain FA. Despite this, higher (P<0.05) contents of polyunsaturated fatty acids (PUFA) and linoleic acid were found in the C7 treatment compared to the C5 treatment (2.48 and 2.22 vs. 2.16 and 1.92 g/100 g of FA methyl es ters, respectively). Including the high levels of cottonseed and barley as a concentrate source for feeding dairy cattle revealed similar MY and milk fat content. However, the high level of cottonseed in the diet of dairy cows showed decreased milk protein content and increased linoleic acid and PUFA levels. PB Islamic Azad University SN 2251-628X YR 2011 FD 2011-12-01 LK https://hdl.handle.net/10347/39236 UL https://hdl.handle.net/10347/39236 LA eng NO Roca-Fernández, A. I., González-Rodríguez, A., Vázquez-Yáñez, O. P. & Fernández-Casado, J. A. (2011). Effects of Concentrate Source (Cottonseed vs. Barley) on Milk Performance and Fatty Acids Profile of Holstein-Friesian Dairy Cows. Iranian Journal of Applied Animal Science 1(4), 245-252. https://sanad.iau.ir/en/Article/1023551 NO Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria. RTA2005-00204-00 [Evaluación de la eficacia de sistemas sostenibles de producción de leche a través de la aplicación de sistemas de soporte de decisiones y de metodologías prácticas de estimación de la ingestión de nutrientes en explotaciones comerciales de Galicia] DS Minerva RD 24 abr 2026