RT Journal Article
T1 Fractionation and purification of bioactive compounds obtained from a brewery waste stream
A1 Barbosa Pereira, Letricia
A1 Pocheville, Ainara
A1 Angulo, Inmaculada
A1 Paseiro Losada, Perfecto
A1 Cruz Freire, José Manuel
K1 Stream
K1 Waste
K1 Bioactive phenolic compounds
AB The brewery industry generates waste that could be used to yield a natural extract containing bioactive phenolic compounds. We compared two methods of purifying the crude extract—solid-phase extraction (SPE) and supercritical fluid extraction (SFE)—with the aim of improving the quality of the final extract for potential use as safe food additive, functional food ingredient, or nutraceutical. The predominant fractions yielded by SPE were the most active, and the fraction eluted with 30% (v/v) of methanol displayed the highest antioxidant activity (0.20 g L−1), similar to that of BHA. The most active fraction yielded by SFE (EC50 of 0.23 g L−1) was obtained under the following conditions: temperature 40∘C, pressure 140 bar, extraction time 30 minutes, ethanol (6%) as a modifier, and modifier flow 0.2 mL min−1. Finally, we found that SFE is the most suitable procedure for purifying the crude extracts and improves the organoleptic characteristics of the product: the final extract was odourless, did not contain solvent residues, and was not strongly coloured. Therefore, natural extracts obtained from the residual stream and purified by SFE can beused as natural antioxidants with potential applications in the food, cosmetic, and pharmaceutical industries
PB Hindawi
SN 2314-6133
YR 2013
FD 2013
LK http://hdl.handle.net/10347/21480
UL http://hdl.handle.net/10347/21480
LA eng
NO Barbosa-Pereira, Letricia, Pocheville, Ainara, Angulo, Inmaculada, Paseiro-Losada, Perfecto, Cruz, José M. (2013). Fractionation and Purification of Bioactive Compounds Obtained from a Brewery Waste Stream. "Biomed research international", vol. 2013
NO This work was financially supported by the Consorcios Estrategicos Nacionales en Investigación Técnica (CENIT) of Ministerio de Ciencia y Inovacion (CENIT-2007-2016-FUTURAL). The authors are grateful to the Mahou-San Miguel Group and FUTURAL project (Ingenio ProgramCDTI)
DS Minerva
RD 27 abr 2026