RT Journal Article
T1 Biotransformation of organic micropollutants by anaerobic sludge enzymes
A1 González Gil, Lorena
A1 Krah, Daniel
A1 Ghattas, Ann-Kathrin
A1 Carballa Arcos, Marta
A1 Wick, Arne
A1 Helmholz, Lissa
A1 Lema Rodicio, Juan Manuel
A1 Ternes, Thomas A.
K1 Anaerobic digestion
K1 Degradation
K1 Enzymatic transformation
K1 Enzymatic pathways
K1 Pharmaceuticals
K1 Sewage treatment plant
AB Biotransformation of organic micropollutants (OMPs) in wastewater treatment plants ultimately depends on the enzymatic activities developed in each biological process. However, few research efforts have been made to clarify and identify the role of enzymes on the removal of OMPs, which is an essential knowledge to determine the biotransformation potential of treatment technologies. Therefore, the purpose of the present study was to investigate the enzymatic transformation of 35 OMPs under anaerobic conditions, which have been even less studied than aerobic systems. Initially, 13 OMPs were identified to be significantly biotransformed (>20%) by anaerobic sludge obtained from a full-scale anaerobic digester, predestining them as potential targets of anaerobic enzymes. Native enzymes were extracted from this anaerobic sludge to perform transformation assays with the OMPs. In addition, the effect of detergents to recover membrane enzymes, as well as the effects of cofactors and inhibitors to promote and suppress specific enzymatic activities were evaluated. In total, it was possible to recover enzymatic activities towards 10 out of these 13 target OMPs (acetyl-sulfamethoxazole and its transformation product sulfamethoxazole, acetaminophen, atenolol, clarithromycin, citalopram, climbazole, erythromycin, and terbutryn, venlafaxine) as well as towards 8 non-target OMPs (diclofenac, iopamidol, acyclovir, acesulfame, and 4 different hydroxylated metabolites of carbamazepine). Some enzymatic activities likely involved in the anaerobic biotransformation of these OMPs were identified. Thereby, this study is a starting point to unravel the still enigmatic biotransformation of OMPs in wastewater treatment systems
PB Elsevier
SN 0043-1354
YR 2019
FD 2019-04-01
LK http://hdl.handle.net/10347/18325
UL http://hdl.handle.net/10347/18325
LA eng
NO Gonzalez-Gil, L., Krah, D., Ghattas, A., Carballa, M., Wick, A., & Helmholz, L. et al. (2019). Biotransformation of organic micropollutants by anaerobic sludge enzymes. Water Research, 152, 202-214. doi: 10.1016/j.watres.2018.12.064
NO This is the accepted manuscript of the following article: Gonzalez-Gil, L., Krah, D., Ghattas, A., Carballa, M., Wick, A., & Helmholz, L. et al. (2019). Biotransformation of organic micropollutants by anaerobic sludge enzymes. Water Research, 152, 202-214. doi: 10.1016/j.watres.2018.12.064
NO This research was funded by the European Research Council (ERC) through the EU-Project ATHENE (Grant agreement 267897), by a Short Term Scientific Mission of the Water2020 Cost Action ES1202, by a FPU Grant (FPU13/01255) and by the COMETT project (CTQ2016-80847-R). Authors from Universidade de Santiago de Compostela belong to CRETUS Strategic Partnership (AGRUP2015/02) and to Galicia Competitive Research Group (GRC ED431C 2017/29), programs co-funded by FEDER (EU)
DS Minerva
RD 24 abr 2026