RT Journal Article
T1 Killing bacteria by faradaic processes through Nano-Hydroxyapatite/MoOx platforms
A1 Sieben, Juan M.
A1 Placente, Damián
A1 Baldini, Mónica D.
A1 Ruso Beiras, Juan Manuel
A1 Laiuppa, Juan A.
A1 Santillán, Graciela E.
A1 Messina, Paula V.
K1 Hydroxyapatite
K1 MoOx
K1 Cyclic voltammetry
K1 Chronoamperometry
K1 Discrete wavelet transform
K1 Drug-independent antibiotic system
AB Following the secular idea of ″restitutio ad integrum″, regeneration is the pursued option to restore bones lost after a disease; accordingly, complementing antibiotic and regeneration capacity to bone grafts represents a great scientific success. This study is a framework proposal for understanding the antimicrobial effect of biocompatible nano-hydroxyapatite/MoOx (nano-HA/MoOx) platforms on the basis of their electroactive behavior. Through cyclic voltammetry and chronoamperometry measurements, the electron transference capacity of nano-HA and nano-HA/MoOx electrodes was determined in the presence of pathogenic organisms: Pseudomonas aeruginosa and Staphylococcus aureus. Faradaic processes were confirmed and related to the switch of MoO42–/PO43– groups in the original hexagonal nano-HA crystal lattice and to the extent of OH vacancies that act as electron acceptors. Microscopic analysis of bacteria’s ultrastructure showed a disruptive effect on the cytoplasmic membrane upon direct contact with the materials, which is not evident in the presence of eukaryotic cells. Experiments support the existence of a type of extracellular electron transfer (EET) process that alters the function of the bacterial cytoplasmic membrane, accelerating their death. Our findings provide strong quantitative support for a drug-independent biocidal physical approach based on EET processes between microorganisms and phosphate ceramics that can be used to combat local orthopedic infections associated with implants.
PB American Chemical Society
SN 1944-8244
YR 2023
FD 2023-05-19
LK https://hdl.handle.net/10347/44095
UL https://hdl.handle.net/10347/44095
LA eng
NO ACS Appl. Mater. Interfaces 2023, 15, 21, 25884–25897
NO This document is the Accepted Manuscript version of a Published Work that appeared in final form in ACS Applied Materials & Interfaces, copyright © 2023 American Chemical Society after peer review and technical editing by the publisher. To access the final edited and published work see https://doi.org/10.1021/acsami.3c05064
NO The authors acknowledge the financial support of Universidad Nacional del Sur (UNS, PGI 24/Q092), Ministerio de Ciencia e Innovación (PID2019- 805 111327GB-100), and Xunta de Galicia (ED431B 2022/36). D.P. thanks CONICET for his fellowship. J.M.S., G.E.S., and P.V.M. are researchers of CONICET.
DS Minerva
RD 28 abr 2026