RT Journal Article
T1 Stimuli-responsive selection of target DNA sequences by synthetic bZIP peptides
A1 Mosquera Mosquera, Jesús
A1 Jiménez Balsa, Adrián
A1 Dodero, Verónica Isabel
A1 Vázquez Sentís, Marco Eugenio
A1 Mascareñas Cid, José Luis
K1 DNA
K1 Synthetic bZIP peptides
AB One of the strategies used by nature to regulate gene expression relies on the stimulicontrolled combination of DNA-binding proteins. This in turn determines the target-binding site within the genome, and thereby whether a particular gene is activated or repressed. Here we demonstrate how a designed basic region leucine zipper-based peptide can be directed towards two different DNA sequences depending on its dimerization arrangement. While the monomeric peptide is non-functional, a C-terminal metallo-dimer recognizes the natural ATF/CREB-binding site (50 -ATGA cg TCAT-30 ), and a N-terminal disulphide dimer binds preferentially to the swapped sequence (50 -TCAT cg ATGA-30 ). As the dimerization mode can be efficiently controlled by appropriate external reagents, it is possible to reversibly drive the peptide to either DNA site in response to such specific inputs. This represents the first example of a designed molecule that can bind to more than one specific DNA sequence depending on changes in its environment.
PB Nature Publishing Group
SN 2041-1723
YR 2013
FD 2013
LK http://hdl.handle.net/10347/22210
UL http://hdl.handle.net/10347/22210
LA eng
NO Mosquera Mosquera, J., Jimenez Balsa, A., Dodero, V.I., Vázquez Sentís, M.E. y Mascareñas Cid, J.L.(2013). Stimuli-responsive selection of target DNA sequences by synthetic bZIP peptides. Nat. commun., vol.4:1874
NO We thank the support given by the Spanish grants SAF2010-20822-C02, CTQ2012-31341, CSD2007-00006, Consolider Ingenio 2010, and the Xunta de Galicia INCITE09209 084PR, GRC2010/12, PGIDIT08CSA-047209PR. J.M. and A.J-B. thank the Spanish Ministry of Education for their PhD fellowships
DS Minerva
RD 23 abr 2026