RT Dissertation/Thesis
T1 Limitations of 16S rRNA gene as phylogenetic marker: a large-scale meta-omics analysis of plaque microbiota in periodontal diseases
A1 Regueira Iglesias, Alba
K1 16S rRNA gene
K1 oral microbiota
K1 matching amplicon
K1 coverage
K1 OTU clustering
K1 periodontal diseases
K1 dental plaque
AB In the literature, 16S rRNA gene sequencing is the most widely usedtechnology for studying the periodontal microbiota. However, there is no evidence on how methodological aspectssuch as primer coverage, detection of matching amplicons (MAs), and clustering into operational taxonomic units(OTUs) could influence the results obtained for the oral niche.Furthermore, the comparison of 16S sequencing-based studies on periodontal microbiota is controversial due tosignificant methodological differences. Therefore, meta-omics analyses would favour the accuracy of phylogeneticdata associated with different periodontal conditions. In the present Thesis, we analysed in silico 1) the coverage ofprimers employed in sequencing-based studies of the mouth microbiota using oral-specific databases containingbacterial and archaeal 16S rRNA gene sequences; 2) the number of 16S rRNA genes in the complete genomes ofbacterial and archaeal species inhabiting the human mouth, and how the use of different primers would affect thedetection of MAs from different taxa; and 3) the performance of different primers to detect distinct oral species with16S rRNA gene amplicon similarity ≥97%, identifying the taxa that may be erroneously grouped into thesame OTU.
YR 2022
FD 2022
LK http://hdl.handle.net/10347/29308
UL http://hdl.handle.net/10347/29308
LA eng
DS Minerva
RD 28 abr 2026