RT Journal Article
T1 Experimental susceptibility of European sea bass and Senegalese sole to different betanodavirus isolates
T2 Susceptibilidad experimental de la lubina europea y el lenguado senegalés a diferentes aislados de betanodavirus
A1 Souto Pereira, Sandra
A1 López Jimena, Benjamín
A1 Alonso, Maria Carmen
A1 García-Rosado, Esther
A1 Bandín Matos, Isabel
K1 Betanodavirus
K1 European sea bass
K1 Experimental infection
K1 Genotypes
K1 Natural reassortment
K1 Senegalese sole
AB The susceptibility of juvenile European sea bass and Senegalese sole to three VNNV isolates (a reassortant RGNNV/SJNNV, as well as the parental RGNNV and SJNNV genotypes) has been evaluated by challenges using two inoculation ways (bath and intramuscular injection). The results demonstrate that these two fish species are susceptible to all the VNNV isolates tested. In European sea bass, RGNNV caused the highest cumulative mortality, reaching maximum values of viral RNA and titres. Although the SJNNV isolate did not provoke mortality or clinical signs of disease in this fish species, viral production in survivor fish was determined; on the other hand the reassortant isolate did cause mortality and clinical signs of disease, although less evident than those recorded after RGNNV infection. These results suggest that the changes suffered by the SJNNV RNA2 segment of the reassortant isolate, compared to the parental SJNNV, may have involved host-specificity and/or virulence determinants for European sea bass. Regarding Senegalese sole, although the three isolates caused 100% mortality, the reassortant strain provoked the most acute symptoms, and more quickly, especially in the bath challenge. This was also the isolate showing less difference between the number of RNA copies and viral titre, reaching the highest titres of infective viral particles in nervous tissue of infected animals. The RGNNV isolate produced the lowest values of infective viral particles. All these results suggest that the RGNNV and the reassortant isolates are the most suited for infecting European sea bass and Senegalese sole, respectively.
PB Elsevier
SN 1873-2542
YR 2015
FD 2015-03-04
LK https://hdl.handle.net/10347/45976
UL https://hdl.handle.net/10347/45976
LA eng
NO Souto S, Lopez-Jimena B, Alonso MC, García-Rosado E, Bandín I. Experimental susceptibility of European sea bass and Senegalese sole to different betanodavirus isolates. Vet Microbiol. 2015 May 15;177(1-2):53-61. doi: 10.1016/j.vetmic.2015.02.030. Epub 2015 Mar 4. PMID: 25770892.
NO Ayuda EM2012/ 005 de la Xunta de Galicia, Novartis Animal Health Inc. (Canada).
DS Minerva
RD 28 abr 2026