RT Journal Article
T1 Full-length hybrid transcriptome of the olfactory rosette in Senegalese sole (Solea senegalensis): an essential genomic resource for improving reproduction on farms
A1 Torres Sabino, Dorinda
A1 Blanco Hortas, Andrés
A1 Rodríguez Villamayor, Paula
A1 Rasines, Inmaculada
A1 Martín, Ignacio
A1 Bouza Fernández, María Carmen
A1 Robledo Sánchez, Diego
A1 Martínez Portela, Paulino
K1 Senegalese sole
K1 Hybrid transcriptome
K1 Olfactory rosette
K1 Olfactory receptor gene families
AB Senegalese sole is a promising European aquaculture species whose main challenge is that captive-born males (F1) are unable to reproduce in farms, hindering breeding programs. Chemical communication through the olfactory system is hypothesized to stem this issue. Although significant advancement in genomic resources has been made recently, scarce information exists on the genomic basis of olfaction, a special sensory system for demersal species like flatfish, which could play a prominent role in reproduction, social and environmental interactions. A full-length transcriptome of the olfactory rosettes including females, males, juveniles and adults, of both F1 and wild origins, was generated at the isoform-level by combining Oxford Nanopore long-read and Illumina short-read sequencing. A total of 20,670 transcripts actively expressed were identified: 13,941 known transcripts, 5,758 novel transcripts from known genes, and 971 from novel genes. Given the important role of olfaction in reproductive behaviour, we comparatively examined the expression and functional enrichment of the olfactory receptor gene families (OlfC, OR, ORA, and TAAR). Our comprehensive olfactory transcriptome of Senegalese sole provides a foundation for delving into the functional basis of this complex organ in teleost and flatfish. Furthermore, it provides a valuable resource for addressing reproductive management challenges in Senegalese sole aquaculture
PB Oxford Academic
SN 1340-2838
YR 2025
FD 2025-10-07
LK https://hdl.handle.net/10347/46388
UL https://hdl.handle.net/10347/46388
LA eng
NO Torres-Sabino, D., Blanco-Hortas, A., Villamayor, P. R., Rasines, I., Martín, I., Bouza, C., Robledo, D., & Martínez, P. (2025). Full-length hybrid transcriptome of the olfactory rosette in Senegalese sole ( Solea senegalensis ): an essential genomic resource for improving reproduction on farms. Oxford University Press (OUP). https://10.1093/dnares/dsaf028
DS Minerva
RD 23 may 2026