RT Journal Article
T1 Long-amplicon MinION-based sequencing study in a salt-contaminated twelfth century granite-built chapel
A1 Pavlovic, Jelena
A1 Bosch Roig, Pilar
A1 Rusková, Magdaléna
A1 Planý, Matej
A1 Pangallo, Domenico
A1 Sanmartín Sánchez, Patricia
K1 Long amplicons
K1 MinION sequencing
K1 Salt contamination
K1 Stone
K1 Batrachochytrium
K1 Bio-desalination
AB The irregular damp dark staining on the stonework of a salt-contaminated twelfth century granite-built chapel is thought to be related to a non-homogeneous distribution of salts and microbial communities. To enhance understanding of the role of microorganisms in the presence of salt and damp stains, we determined the salt content and identified the microbial ecosystem in several paving slabs and inner wall slabs (untreated and previously bio-desalinated) and in the exterior surrounding soil. Soluble salt analysis and culture-dependent approaches combined with archaeal and bacterial 16S rRNA and fungal ITS fragment as well as with the functional genes nirK, dsr, and soxB long-amplicon MinION-based sequencing were performed. State-of-the-art technology was used for microbial identification, providing information about the microbial diversity and phylogenetic groups present and enabling us to gain some insight into the biological cycles occurring in the community key genes involved in the different geomicrobiological cycles. A well-defined relationship between microbial data and soluble salts was identified, suggesting that poorly soluble salts (CaSO4) could fill the pores in the stone and lead to condensation and dissolution of highly soluble salts (Ca(NO3)2 and Mg(NO3)2) in the thin layer of water formed on the stonework. By contrast, no direct relationship between the damp staining and the salt content or related microbiota was established. Further analysis regarding organic matter and recalcitrant elements in the stonework should be carried out
PB Springer
SN 0175-7598
YR 2022
FD 2022
LK http://hdl.handle.net/10347/29201
UL http://hdl.handle.net/10347/29201
LA eng
NO Applied Microbiology and Biotechnology 106, 4297–4314 (2022). https://doi.org/10.1007/s00253-022-11961-8
NO Open Access funding provided thanks to the CRUE-CSIC agreement with Springer Nature. P. Sanmartín acknowledges receipt of a Ramón y Cajal contract (RYC2020-029987-I) financed by the Spanish Ministry of Science and Innovation (MICINN). The authors acknowledge the projects APVV-19–0059 and VEGA 2/099/2021 which also financed this study. The authors acknowledge CONSORCIO DE LA CIUDAD DE SANTIAGO funding for this research
DS Minerva
RD 23 abr 2026