RT Journal Article
T1 Evidence for the role of extrusomes in evading attack by the host immune system in a scuticociliate parasite
A1 Folgueira López, Iria
A1 Lamas Fernández, Jesús
A1 Felipe González, Ana Paula de
A1 Sueiro Benavides, Rosa Ana
A1 Leiro Vidal, José Manuel
K1 Philasterides dicentrarchi
K1 Turbot
K1 Exocytosis
K1 Extrusomes
K1 Trichocyst matrix proteins
K1 Mucin-like glycoproteins
AB Like other ciliates, Philasterides dicentrarchi, the scuticociliate parasite of turbot, produces a feeding-only or growing stage called a trophont during its life cycle. Exposure of the trophonts to heat-inactivated serum extracted from the turbot host and containing specific antibodies that induce agglutination/immobilization leads to the production of a mucoid capsule from which the trophonts later emerge. We investigated how these capsules are generated, observing that the mechanism was associated with the process of exocytosis involved in the release of a matrix material from the extrusomes. The extruded material contains mucin-like glycoproteins that were deposited on the surface of the cell and whose expression increased with time of exposure to the heat-inactivated immune serum, at both protein expression and gene expression levels. Stimulation of the trophonts with the immune serum also caused an increase in discharge of the intracellular storage compartments of calcium necessary for the exocytosis processes in the extrusomes. The results obtained suggest that P. dicentrarchi uses the extrusion mechanism to generate a physical barrier protecting the ciliate from attack by soluble factors of the host immune system. Data on the proteins involved and the potential development of molecules that interfere with this exocytic process could contribute to improving the prevention and control of scuticociliatosis in turbot
PB Elsevier
SN 1050-4648
YR 2019
FD 2019
LK http://hdl.handle.net/10347/19714
UL http://hdl.handle.net/10347/19714
LA eng
NO Folgueira I, Lamas J, De Felipe AP, Sueiro RA, Leiro JM. (2019). Evidence for the role of extrusomes in evading attack by the host immune system in a scuticociliate parasite. Fish Shellfish Immunol. 2019 Jul 5;92:802-812. doi: 10.1016/j.fsi.2019.07.008
NO This is the accepted manuscript of the following article: Folgueira I, Lamas J, DeFelipe AP, Sueiro RA, Leiro JM. (2019). Evidence for the role of extrusomes inevading attack by the host immune system in a scuticociliate parasite. FishShellfish Immunol. 2019 Jul 5;92:802-812. doi: 10.1016/j.fsi.2019.07.008
NO This study was financially supported by grants from the Ministerio de Economía y Competitividad and Fondo Europeo de Desarrollo Regional -FEDER- (European Union) (AGL2017-83577-R) and from the Xunta de Galicia (ED431C2017/31) and also by the PARAFISHCONTROL project, which received funding from the European Union's Horizon 2020 research and innovation programme under grant agreement No. 634429
DS Minerva
RD 24 abr 2026