RT Journal Article
T1 A novel saliva-based miRNA signature for colorectal cancer diagnosis
A1 Rapado González, Óscar
A1 Majem, Blanca
A1 Álvarez Castro, Ana
A1 Díaz Peña, Roberto
A1 Abalo, Alicia
A1 Suárez Cabrera, Leticia
A1 Gil Moreno, Antonio
A1 Santamaría, Anna
A1 López López, Rafael
A1 Muinelo Romay, Laura
A1 Suárez Cunqueiro, María Mercedes
A1 Caínzos Fernández, Miguel Ángel
K1 Liquid biopsy
K1 Saliva
K1 Colorectal cancer
K1 MicroRNA
K1 Circulating biomarkers
K1 Epigenomics
AB Salivary microRNAs (miRNAs) are of high interest as diagnostic biomarkers for non-oralcancer. However, little is known about their value for colorectal cancer (CRC) detection. Our studyaims to characterize salivary miRNAs in order to identify non-invasive markers for CRC diagnosis.The screening of 754 miRNAs was performed in saliva samples from 14 CRC and 10 healthycontrols. The di erential expressed miRNAs were validated by RT-qPCR in 51 CRC, 19 adenomasand 37 healthy controls. Receiver operating characteristic (ROC) curves and logistic regressionmodels were performed to analyze the clinical value of these miRNAs. Twenty-two salivary miRNAswere significantly deregulated in CRC patients vs. healthy individuals (p < 0.05) in the discoveryphase. From those, five upregulated miRNAs (miR-186-5p, miR-29a-3p, miR-29c-3p, miR-766-3p, andmiR-491-5p) were confirmed to be significantly higher in the CRC vs. healthy group (p < 0.05). Thisfive-miRNA signature showed diagnostic value (72% sensitivity, 66.67% specificity, AUC = 0.754)to detect CRC, which was even higher in combination with carcinoembryonic antigen (CEA) levels.Overall, after the first global characterization of salivary miRNAs in CRC, a five-miRNA panel wasidentified as a promising tool to diagnose this malignancy, representing a novel approach to detectcancer-associated epigenetic alterations using a non-invasive strategy
PB MDPI
YR 2019
FD 2019
LK http://hdl.handle.net/10347/21254
UL http://hdl.handle.net/10347/21254
LA eng
NO Rapado-González, Ó.; Majem, B.; Álvarez-Castro, A.; Díaz-Peña, R.; Abalo, A.; Suárez-Cabrera, L.; Gil-Moreno, A.; Santamaría, A.; López-López, R.; Muinelo-Romay, L.; Suarez-Cunqueiro, M.M. A Novel Saliva-Based miRNA Signature for Colorectal Cancer Diagnosis. J. Clin. Med. 2019, 8, 2029
NO This research was funded by Eugenio Rodríguez Pascual Foundation and the Galician society thatparticipated in the Liquid Biopsy crowdfunding. L.M.-R is supported by AECC Foundation
DS Minerva
RD 24 abr 2026