RT Journal Article
T1 Optimization of a miniaturized solid-phase microextraction method followed by gas chromatography mass spectrometry for the determination of twenty four volatile and semivolatile compounds in honey from Galicia (NW Spain) and foreign countries
A1 Vázquez Ferreiro, Lúa
A1 Celeiro Montero, María
A1 Sergazina, Meruyert
A1 Dagnac, Thierry
A1 Llompart Vizoso, María del Pilar
K1 Honey
K1 Miniaturized solid phase microextraction
K1 Experimental design
K1 GC-MS
AB An analytical miniaturized methodology based on solid-phase-microextraction (mini_SPME) followed by gas chromatography coupled to mass spectrometry (GC-MS) has been developed for the identification of volatile and semivolatile compounds in honey samples. The main influential experimental parameters, such as the type of fibre coating, extraction temperature, solvent addition, extraction mode, ionic strength, and sample dilution were optimized. A design of experiments (DOE) was conducted including twenty-four target compounds. The final extraction conditions comprised the use of 200 mg of honey mixed with 200 μL of water (100%, w/v), employing a DVB/CAR/PDMS fibre in the headspace mode at 100 °C for 30 min. The mini_SPME-GC-MS method was successfully validated in terms of linearity, repeatability, reproducibility and accuracy. Finally, it was applied to a broad range of varieties of real honey samples from Galicia (NW Spain), as well as some foreign honeys, demonstrating suitability
PB Elsevier
SN 2352-5541
YR 2021
FD 2021
LK http://hdl.handle.net/10347/26253
UL http://hdl.handle.net/10347/26253
LA eng
NO Sustainable Chemistry and Pharmacy, 21 (2021), 100451
NO This research was supported by GO project FEADER 2018/054B. The authors belong to the National Network for the Innovation in miniaturized sample preparation techniques, RED2018-102522-T (Ministry of Science, Innovation and Universities, Spain), to the Galician Competitive Research Groups IN607B 2019/13 and ED431 2020/06 and to the CRETUS Strategic Partnership ED431 2018/01 (Xunta de Galicia)
DS Minerva
RD 24 abr 2026