RT Journal Article
T1 Combined (d)SPE-QuEChERS Extraction of Mycotoxins in Mixed Feed Rations and Analysis by High Performance Liquid Chromatography-High-Resolution Mass Spectrometry
A1 Facorro Souto, Rocío
A1 Llompart Vizoso, María del Pilar
A1 Dagnac, Thierry
K1 Mycotoxins
K1 Mixed feed rations
K1 QuEChERS
K1 Dispersive solid phase extraction
K1 Liquid chromatography
K1 High-resolution mass spectrometry
K1 Data independent SWATH
AB The objective of this work was the development of a methodology capable of simultaneously determine 26 mycotoxins in mixed feed rations collected in 20 dairy farms. A sample preparation methodology based on a combination of (d)SPE and QuEChERS extractions was used. Liquid chromatography-high resolution mass spectrometry was employed for both identification and quantification purposes. To this respect, a powerful workflow based on data-independent acquisition, consisting of fragmenting all precursor ions entering the mass spectrometer in narrow m/z isolation windows (SWATH), was implemented. SWATH data file then contains all the information that would be acquired in a multitude of different experimental approaches in a single all-encompassing dataset. Analytical method performance was evaluated in terms of linearity, repeatability and matrix effect. Relative recoveries were also measured, giving values above 80% for most compounds. Matrix-matched calibration was carried out and enabled reaching the low ng mL−1 level for many mycotoxins. The observed matrix effect, in most cases suppressive, reached even values higher than 60%. The repeatability was also adequate, showing a relative standard deviation lower than 10%. All unified samples analyzed showed co-occurrence of two or more mycotoxins, recurrently zearalenone, fumonisin B1, and β-zearalenol, with an occurrence frequency ranging from 60% to 90%
PB MDPI
YR 2020
FD 2020
LK http://hdl.handle.net/10347/23410
UL http://hdl.handle.net/10347/23410
LA eng
NO Facorro, R.; Llompart, M.; Dagnac, T. Combined (d)SPE-QuEChERS Extraction of Mycotoxins in Mixed Feed Rations and Analysis by High Performance Liquid Chromatography-High-Resolution Mass Spectrometry. Toxins 2020, 12, 206
NO This work was supported by the CONECTA-PEME GALAC project IN852A 2016/103. This work was also supported by the project IGDC13-1E-2026 (Infrastructure Program, MINECO, Spain). The authors belong to the Galician Competitive Research Groups GPC 2017/04 and GPC IN607B 2019/13, and to the CRETUS Strategic Partnership (ED431E 2018/01). All these programs are co-funded by FEDER (UE)
DS Minerva
RD 29 abr 2026