RT Journal Article
T1 Integration of maps enables a cytogenomics analysis of the complete karyotype in solea senegalensis
A1 Ramírez, Daniel
A1 Rodríguez, María Esther
A1 Alejandro Merlo, Manuel
A1 Anaya, Marco
A1 Portela-Bens, Silvia
A1 Martinez Portela, Paulino
A1 Robles, Francisca
A1 Rebordinos, Laureana
A1 Cross, Ismael
A1 Arias Pérez, Alberto
A1 Ruíz Rejón, Carmelo
K1 Solea senegalensis
K1 Pleuronectiformes
K1 Genetic maps
K1 Cytogenomics
K1 Chromosome evolution
K1 Karyotype
K1 Repetitive sequences
K1 Comparative genomics
AB The Pleuronectiformes order, which includes several commercially-important species, has undergone extensive chromosome evolution. One of these species is Solea senegalensis, a flatfish with 2n = 42 chromosomes. In this study, a cytogenomics approach and integration with previous maps was applied to characterize the karyotype of the species. Synteny analysis of S. senegalensis was carried out using two flatfish as a reference: Cynoglossus semilaevis and Scophthalmus maximus. Most S. senegalensis chromosomes (or chromosome arms for metacentrics and submetacentrics) showed a one-to-one macrosyntenic pattern with the other two species. In addition, we studied how repetitive sequences could have played a role in the evolution of S. senegalensis bi-armed (3, and 5–9) and acrocentric (11, 12 and 16) chromosomes, which showed the highest rearrangements compared with the reference species. A higher abundance of TEs (Transposable Elements) and other repeated elements was observed adjacent to telomeric regions on chromosomes 3, 7, 9 and 16. However, on chromosome 11, a greater abundance of DNA transposons was detected in interstitial BACs. This chromosome is syntenic with several chromosomes of the other two flatfish species, suggesting rearrangements during its evolution. A similar situation was also found on chromosome 16 (for microsatellites and low complexity sequences), but not for TEs (retroelements and DNA transposons). These differences in the distribution and abundance of repetitive elements in chromosomes that have undergone remodeling processes during the course of evolution also suggest a possible role for simple repeat sequences in rearranged regions.
PB MDPI
SN 1661-6596
YR 2022
FD 2022
LK http://hdl.handle.net/10347/32380
UL http://hdl.handle.net/10347/32380
LA eng
NO Ramírez, D., Rodríguez, M.E., Cross, I., Arias-Pérez, A., Alejandro Merlo, M., Anaya, M., Portela-Bens, S., Martínez, P., Robles, F., Ruiz-Rejón, C. And Rebordinos, L. (2022). Integration of maps enables a cytogenomics analysis of the complete karyotype in solea senegalensis. International journal of molecular sciences, 23(10), 5353. https://doi.org/10.3390/ijms23105353
NO This study was supported by the Spanish Ministry of Economy and Competitiveness, FEDER Grants (RTI2018-096847-B-C21 and RTI2018-096847-B-C22), Junta de Andalucía-FEDER Grant (P20-00938).
DS Minerva
RD 24 abr 2026