RT Journal Article
T1 Study of protein xxpresion in peri-infarct tissue after cerebral ischemia
A1 Brea López, David
A1 Agulla Freire, Jesús
A1 Staes, An
A1 Gevaert, Kris
A1 Campos Pérez, Francisco
A1 Sobrino Moreiras, Tomás
A1 Blanco González, Miguel
A1 Dávalos, Antoni
A1 Castillo Sánchez, José Antonio
A1 Ramos Cabrer, Pedro
AB In this work, we report our study of protein expression in rat peri-infarct tissue, 48h after theinduction of permanent focal cerebral ischemia. Two proteomic approaches, gel electrophoresis withmass spectrometry and combined fractional diagonal chromatography (COFRADIC), were performedusing tissue samples from the periphery of the induced cerebral ischemic lesions, using tissue fromthe contra-lateral hemisphere as a control. Several protein spots (3408) were identified by gelelectrophoresis, and 11 showed significant differences in expression between peri-infarct and contralateral tissues (at least 3-fold, p<0.05). Using COFRADIC, 5412 proteins were identified, with 72showing a difference in expression. Apart from blood-related proteins (such as serum albumin), bothtechniques showed that the 70kDa family of heat shock proteins were highly expressed in the periinfarct tissue. Further studies by 1D and 2D western blotting and immunohistochemistry revealedthat only one member of this family (the inducible form, HSP72 or HSP70i) is specifically expressedby the peri-infarct tissue, while the majority of this family (the constitutive form, HSC70 or HSP70c)is expressed in the whole brain. Our data support that HSP72 is a suitable biomarker of peri-infarcttissue in the ischemic brain.
PB Nature Publishing Group
YR 2015
FD 2015
LK http://hdl.handle.net/10347/21731
UL http://hdl.handle.net/10347/21731
LA eng
NO Brea, D. et al. Study of Protein Expresion in Peri-Infarct Tissue after Cerebral Ischemia. Sci. Rep. 5, 12030; doi: 10.1038/srep12030 (2015).
NO The authors are grateful to the Instituto de Salud Carlos III (PI11/02161, CP09/00074 and RETICSR012/0014), the Spanish Ministry of Economy (SAF2008-02190) and the European Union (FEDERprogram), for financial support. PRC and TS are the recipients of a research contract from the MiguelServet program, and DB and JA were the recipients of a research contract from the Sara Borrell programfrom the Instituto de Salud Carlos III of the Spanish Ministry of Health.
DS Minerva
RD 28 abr 2026