RT Journal Article
T1 Enrichment of nitrite-dependent anaerobic methane oxidizing bacteria in a membrane bioreactor
A1 Allegue Martínez, Tomás Francisco
A1 Fernández González, Nuria
A1 Omil Prieto, Francisco
A1 Garrido Fernández, Juan Manuel
K1 Methanogenic reactors
K1 N-damo bacteria
K1 Dissolved methane
K1 Nitrous oxide
K1 Ammonium and membrane bioreactor
AB The use of nitrite-dependent anaerobic methane oxidation (n-damo) processes could represent an innovative technology in order to minimize the environmental impact of anaerobic sewage effluents at low temperatures, since these biological processes are able to simultaneously remove nitrite and dissolved methane in anaerobic conditions. Nevertheless, n-damo bacteria are well-known by their reported low activity and slow doubling times which hinders a practical application. On this study, the enrichment on these bacteria was successfully achieved in a membrane bioreactor system at 28 °C. Despite biomass accumulation was not detected, a high apparent specific n-damo activity of 95.5 mg NO2−-N g−1 MLVSS d−1 was achieved after 388 days of operation, being one of the highest nitrite removal rates reported in the literature for n-damo cultures to date. Additionally a slow doubling time of 11.5 d was estimated. 16S rRNA gene amplicon sequencing analysis indicated that Candidatus Methylomirabilis became the most abundant bacterial organism by day 344 with a relative abundance of 50.2%. During the entire experiment ammonium was continuously added to the system as an alternative nitrogen source, to avoid biomass growth limitations. Finally, a relation between permeate nitrite concentrations and nitrous oxide production was found, which allows to optimize the process in terms of the minimization of both nitrogen species. The nitrous oxide emissions represented between 0 and 3.7% of the denitrified nitrogen
PB Elsevier
SN 1385-8947
YR 2018
FD 2018
LK http://hdl.handle.net/10347/25023
UL http://hdl.handle.net/10347/25023
LA eng
NO Chemical Engineering Journal, 347 (2018), 721-730. doi: 10.1016/j.cej.2018.04.134
NO The authors acknowledge the financial support received from Spanish Ministry of Economy and Competitiveness through the projects HOLSIA and COMETT (CTM2013-46750-R & CTQ2016-80847-R), both co-funded by FEDER. T. Allegue would also like to express his gratitude to the same Ministry for awarding a research scholarship (BES-2014-069114). The authors belong to the Galician Competitive Research Group GRC 2013-032, programme co-funded by FEDER, and to CRETUS (AGRUP2015/02)
DS Minerva
RD 8 jun 2026