RT Journal Article
T1 Identification of novel adipokines in the joint. Differential expression in healthy and osteoarthritis tissues
A1 Conde, Javier
A1 Scotece, Morena
A1 Abella, Vanessa
A1 Gómez, Rodolfo
A1 López, Verónica
A1 Villar, Rosa
A1 Hermida Prieto, Miguel
A1 Pino, Jesús
A1 Gómez-Reino Carnota, Juan Jesús
A1 Gualillo, Oreste
AB ObjectivesEmerging data suggest that several metabolic factors, released mainly by white adipose tissue (WAT) and joint tissues, and collectively named adipokines, might have a role in the pathophysiology of OA. Recently, novel adipokines such as SERPINE2, WISP2, GPNMB and ITIH5 have been identified in WAT. The main goal of this study was to analyse the expression of these novel adipokines in synovium, infrapatellar fat pad and chondrocytes and to compare the expression of these molecules in healthy and OA tissues.MethodsSynovial tissues, infrapatellar fat pad and chondrocytes were obtained from 36 OA patients (age 52–85; mean BMI 28.9) who underwent total knee replacement surgery. Healthy synovial tissues and infrapatellar fat pad were obtained from 15 traumatic knee patients (age 23–53; mean BMI 23.5). mRNA and protein expression were determined by qRT-PCR and western blot analysis respectively.ResultsAll the novel adipokines, matter of our study, are expressed in OA synovium, infrapatellar fat pad and chondrocytes. Moreover, we detected a differential expression of SERPINE2 and ITIH5 in OA synovial tissues as compared to healthy samples. Finally, we also observed an increased expression of WISP2 in OA infrapatellar fat pad in comparison to healthy controls.ConclusionsIn this study we demonstrated for the first time the expression of four novel adipokines in different joint tissues and how these molecules are differentially expressed in healthy and OA joint tissues.
PB PLOS
YR 2015
FD 2015
LK http://hdl.handle.net/10347/22180
UL http://hdl.handle.net/10347/22180
LA eng
NO OG is supported by Instituto de Salud Carlos III (PI11/01073) Oreste Gualillo is a member of RETICS Programme, RD12/0009/0008 (RIER:Red de Investigación en Inflamación y Enfermedades Reumáticas) via Instituto de Salud Carlos III (ISCIII). Morena Scotece is a recipient of the “FPU” programme of the Spanish Ministry of Education. Javier Conde is a recipìent of a predoctoral program of Fundación Ramón Domínguez. Vanessa Abella is a predoctoral recipient of Xunta de Galicia. Veronica Lopez is a recipient of a grant from ISCIII
DS Minerva
RD 28 abr 2026