RT Journal Article
T1 Role of surface functionalization and biomolecule structure on protein corona adsorption and conformation onto anisotropic metallic nanoparticles
A1 Figueroa, Valeria
A1 Velasco Rodríguez, Brenda
A1 Arellano, Lilia G.
A1 Domínguez Arca, Vicente
A1 Cambón Freire, Adriana
A1 Pardo Montero, Alberto
A1 Topete Camacho, Antonio
A1 Rosales Rivera, Luis Carlos
A1 Soltero Martinez, José Félix Armando
A1 Barbosa Fernández, Silvia
A1 Taboada Antelo, Pablo
K1 Gold nanorod
K1 Protein corona
K1 Surface functionalization
K1 Cooperative interactions
AB In the biological milieu, nanoparticles (NPs) interact with different biomolecules, particularly proteins, leading to the formation of an interfacial corona, which gives rise to a new biological identity affecting NP biodistribution, cytotoxicity and biological fate. The surface coating of NPs plays a key role in regulating such biocorona formation and composition. We here investigated the interactions between bovine serum albumin (BSA) and bovine fibrinogen (FIB) with gold nanorods (Au NRs) bearing different surface coatings (cetyltrimethylammonium bromide, CTAB, and carboxylic acid and amine-terminated polyethylene glycols (PEGs). It was revealed that CTAB-coated NPs interact with both proteins with high affinity (ca. 108-109 M−1) whereas for PEG ones the extent of protein binding decreases thanks to the stealth properties of PEG, but a protein corona is still formed, with binding affinities between 104-106 M−1. In addition, present results indicated that thicknesses of protein coronas and the aggregation behavior of AuNPs were closely related to their surface properties and protein structure. We also found that BSA and FIB underwent different conformational changes upon adsorption depending on the surface-modified Au NRs. Hence, these findings offered important insights into the essence of the interactions between NPs and proteins toward the development of safe and effective nanomaterials
PB Elsevier
SN 0167-7322
YR 2024
FD 2024
LK http://hdl.handle.net/10347/33882
UL http://hdl.handle.net/10347/33882
LA eng
NO Journal of Molecular Liquids, Volume 398, 2024, 124240
NO Authors thank Agencia Estatal de Investigación (AEI) by project PID2019-109517RB-I00 and Xunta de Galicia for financial support through action ED431C 2022/18. European Regional Development Fund (ERDF) are also acknowledged
DS Minerva
RD 24 abr 2026