RT Journal Article
T1 Bone regeneration in rabbit cranial defects: 3D printed polylactic acid scaffolds gradually enriched with marine bioderived calcium phosphate
A1 Alonso Fernández, Iván
A1 Jostein Haguen, Havard
A1 Parreiras Nogueira, Liebert
A1 López Álvarez, Miriam
A1 González, Pío
A1 López Peña, Mónica
A1 González Cantalapiedra, Antonio
A1 Muñoz Guzón, Fernando María
K1 Polylactic acid
K1 Bioceramic3D-printing technology
K1 Composite scaffolds
K1 Bone regeneration
K1 Rabbit calvarial defect model
AB ObjectiveThis study aimed to evaluate the in vivo biocompatibility, mechanical performance and osteoconductive potential of 3D-printed polylactic acid (PLA) scaffolds enriched with marine bioderived calcium phosphate (bioCaP) for bone tissue engineering.Materials and methodsPLA-bioCaP composite scaffolds were specifically designed for the rabbit cranial defect model by 3D printing, with a uniform distribution of open square-shaped pores and contributions in bioCaP. Physicochemical and mechanical characterization and the evaluation of biological response are presented.ResultsThe scaffolds demonstrated mechanical properties comparable to human bones, integration with the host bone, and osteoconductive behavior promoting cell ingrowth from the defect edge. Strong mineralized tissue ingrowth through the scaffolds’ pores was observed, providing notable support to the host bone. In quantitative terms, micro-CT and histomorphometry analysis post-implantation revealed no significant differences in bone regeneration across all groups.ConclusionThe 3D-printed scaffolds with perpendicular patterning, open porosity, and proposed composition displayed satisfactory mechanical properties, biocompatibility, and osteoconductive response. The scaffolds promoted bone regeneration at similar levels as the PLA. The highest contribution of bioCaP promoted a positive influence in certain histomorphometric parameters; however, it did not significantly improve their osteogenic capability. Further research is required to optimize scaffold composition and enhance their osteogenic potential.Clinical relevanceThis study presents a significant advancement in bone tissue engineering through the development of personalized composite scaffolds for bone-related applications. The clinical implications of this research are profound, especially considering the increasing demand for functional bone regeneration technologies capable of producing cost-effective producing cost-effective customized scaffolds.
PB Elsevier
YR 2024
FD 2024-09-20
LK https://hdl.handle.net/10347/39843
UL https://hdl.handle.net/10347/39843
LA eng
NO References  Fernández, I. A., Haugen, H. J., Nogueira, L. P., Álvarez, M. L., González, P., Peña, M. L., Cantalapiedra, A. G., & Guzón, F. M. (2024). Bone regeneration in rabbit cranial defects: 3D printed polylactic acid scaffolds gradually enriched with marine bioderived calcium phosphate. Materialia, 3810.1016/j.mtla.2024.102240
NO This study was funded by the GRC support program from Xunta de Galicia (GRC ED431C 2021/19 and ED431C 2021/49) and the UE project IBEROS+ (0072_IBEROS_MAIS_1_E), which was financed by the POCTEP 2021-2027 FEDER program.
DS Minerva
RD 24 abr 2026