RT Journal Article
T1 Integrating bioprinting, cell therapies and drug delivery towards in vivo regeneration of cartilage, bone and osteochondral tissue
A1 Abbadessa, Anna
A1 Ronca, Alfredo
A1 Salerno, Aurelio
K1 Bioprinted scafolds 
K1 Bone 
K1 Cartilage 
K1 Osteochondral tissue 
K1 Growth factors 
K1 Spatio-temporal drug release
AB The biological and biomechanical functions of cartilage, bone and osteochondral tissue are naturally orchestrated by a complex crosstalk between zonally dependent cells and extracellular matrix components. In fact, this crosstalk involves biomechanical signals and the release of biochemical cues that direct cell fate and regulate tissue morphogenesis and remodelling in vivo. Three-dimensional bioprinting introduced a paradigm shift in tissue engineering and regenerative medicine, since it allows to mimic native tissue anisotropy introducing compositional and architectural gradients. Moreover, the growing synergy between bioprinting and drug delivery may enable to replicate cell/extracellular matrix reciprocity and dynamics by the careful control of the spatial and temporal patterning of bioactive cues. Although significant advances have been made in this direction, unmet challenges and open research questions persist. These include, among others, the optimization of scaffold zonality and architectural features; the preservation of the bioactivity of loaded active molecules, as well as their spatio-temporal release; the in vitro scaffold maturation prior to implantation; the pros and cons of each animal model and the graft-defect mismatch; and the in vivo non-invasive monitoring of new tissue formation. This work critically reviews these aspects and reveals the state of the art of using three-dimensional bioprinting, and its synergy with drug delivery technologies, to pattern the distribution of cells and/or active molecules in cartilage, bone and osteochondral engineered tissues. Most notably, this work focuses on approaches, technologies and biomaterials that are currently under in vivo investigations, as these give important insights on scaffold performance at the implantation site and its interaction/integration with surrounding tissues
PB Springer
SN 2190-393X
YR 2023
FD 2023
LK http://hdl.handle.net/10347/33029
UL http://hdl.handle.net/10347/33029
LA eng
NO Open Access funding provided thanks to the CRUE-CSIC agreement with Springer Nature. A.A. acknowledges funding from ‘la Caixa’ Foundation (ID 100010434) with a fellowship code LCF/BQ/PR22/11920003
DS Minerva
RD 24 abr 2026