RT Journal Article
T1 A novel strategy for triacylglycerides and polyhydroxyalkanoates production using waste lipids
A1 Argiz Montes, Lucía
A1 González Cabaleiro, Rebeca
A1 Val del Río, Ángeles
A1 González López, Jesús
A1 Mosquera Corral, Anuska
K1 Biotransformation
K1 Lipidic waste
K1 Mixed microbial culture
K1 pH
K1 Uncoupled feeding
AB Lipids are one of the main components of the organic matter present in the effluents of the food-processing industry. These waste streams can be biotransformed into valuable triacylglycerides (TAGs) and polyhydroxyalkanoates (PHAs), precursors of biofuels and biomaterials alternative to petroleum-based products. These compounds are yielded by mixed microbial cultures, and considering that both TAG and PHA accumulators may coexist within the community, it seems crucial to define those operational strategies that might control the selection of the dominant metabolic pathways (TAG or PHA accumulation). In this work, residual fish-canning oil was used as a carbon source in a two-stage process (culture selection and intracellular compounds accumulation) in which the substrate was simultaneously hydrolyzed in these two stages without the need for a previous fermentation unit. It was pretended to maximize preferential TAG or PHA storage in the accumulation reactor by the imposition of certain selective pressures in the enrichment one. Uncoupling C and N feedings and limiting nitrogen availability in the medium, allowed to maximize PHA production (82.3 wt% of PHAs, 0.80 CmmolPHA/CmmolS). Besides, when low pH in the famine phase was considered as additional selective pressure, it was possible to shift the ratio TAG:PHA from 4:96 obtaining 43.0 wt% of TAGs (0.67 CmmolTAG/CmmolS). Therefore, this novel and simplified process demonstrated versatility and efficiency in the storage of TAGs and PHAs from a unique residual feedstock and using an open culture proving that product selection can be harnessed if choosing the right operational conditions in the enrichment stage
PB Elsevier
SN 0048-9697
YR 2021
FD 2021
LK http://hdl.handle.net/10347/25115
UL http://hdl.handle.net/10347/25115
LA eng
NO Science of The Total Environment, 763 (2021), 142944. https://doi.org/10.1016/j.scitotenv.2020.142944
NO This research was supported by the Spanish Government (AEI) through the TREASURE project [CTQ2017-83225-C2-1-R]. Lucía Argiz is a Xunta de Galicia fellow (2019), [ED 481A-2019/083], grant co-funded by the operative program FSE Galicia 2014-2020. Lucía Argiz, Ángeles Val del Río, and Anuska Mosquera-Corral belong to the Galician Competitive Research Group GRC ED431C 2017/29. All these programs are co-funded by the FEDER (EU)
DS Minerva
RD 24 abr 2026