RT Journal Article
T1 Metal-catalyzed uncaging of DNA-binding agents in living cells
A1 Sánchez López, Mateo Isidro
A1 Penas Tallón, Cristina
A1 Vázquez Sentís, Marco Eugenio
A1 Mascareñas Cid, José Luis
AB Attachment of alloc protecting groups to the amidine units of fluorogenic DNA-binding bisbenzamidines or to the amino groups of ethidium bromide leads to a significant reduction of their DNA affinity. More importantly, the active DNA-binding species can be readily regenerated by treatment with ruthenium catalysts in aqueous conditions, even in cell cultures. The catalytic chemical uncaging can be easily monitored by fluorescence microscopy, because the protected products display both different emission properties and cell distribution to the parent compounds
PB Royal Society of Chemistry
YR 2014
FD 2014
LK http://hdl.handle.net/10347/18336
UL http://hdl.handle.net/10347/18336
LA eng
NO Sánchez, M., Penas, C., Vázquez, M., & Mascareñas, J. (2014). Metal-catalyzed uncaging of DNA-binding agents in living cells. Chem. Sci., 5(5), 1901-1907. doi: 10.1039/c3sc53317d
NO We are thankful for the support given by the Spanish grants SAF2010-20822-C02, CTQ2012-31341, Consolider Ingenio 2010 CSD2007-00006, the Xunta de Galicia, GRC2010/12, GRC2013-041, PGIDIT08CSA-047209PR, the ERDF and the European Research Council (Advanced Grant Na 340055). M. I. S. and C. P. thank the Spanish Ministry for their PhD fellowships
DS Minerva
RD 28 abr 2026