RT Journal Article
T1 Optimising medium chain carboxylate production in xylan mixed-culture monofermentation
A1 Iglesias Riobó, Juan
A1 Bonatelli, M. L.
A1 Machado Fernández, C.
A1 Mauricio Iglesias, Miguel
A1 Carballa Arcos, Marta
K1 Chain elongation
K1 Hemicellulose
K1 Caproate
K1 Vitamins
K1 Yeast extract
AB This work investigates the optimization of medium-chain carboxylate (MCC) production through xylan mixed-culture monofermentation. The pH screening in batch assays showed that the hydrolysis stage and selectivity towards MCC precursors were optimised at pH 6. Subsequently, a continuous stirred tank reactor (CSTR) and a Sequential Batch Reactor (SBR) were operated at different Hydraulic Retention Times (HRT), revealing that the SBR at HRT 2 days yielded the highest caproic acid since lactic acid availability and chain elongation process were balanced. An enriched medium with yeast extract and vitamins favoured the growth of chain elongators, and therefore, the MCC production. Moreover, cross-feeding interaction between bacteria in xylan fermentation was observed, and Pseudoramibacter was present in the highest caproic acid yields. This work highlights the impact of selecting the proper operational window to optimise one-stage MCC production.
PB Elsevier
SN 1873-2976
YR 2025
FD 2025-03
LK https://hdl.handle.net/10347/39600
UL https://hdl.handle.net/10347/39600
LA eng
NO Bioresource Technology Volume 420, March 2025, 132124
NO This research was funded by Spanish Government through the Cell4Chem project (PCI2021-121989, ERACoBioTech 3rd call). The authors J. I.-R., M. M.-I., M. C. belong to a Galician Competitive Research Group (ED431C-2021/37). M. L. B. work was carried out as part of the European project Cell4Chem (German funding code: 100572058) that is co-financed by means of taxation based on the budget adopted by the representatives of the Landtag of Saxony.
DS Minerva
RD 26 abr 2026