RT Journal Article
T1 RNA-seq analysis reveals significant transcriptome changes in turbot (Scophthalmus maximus) suffering severe enteromyxosis
A1 Robledo Sánchez, Diego
A1 Ronza, Paolo
A1 Harrison, Peter W.
A1 Losada García, Ana Paula
A1 Bermúdez Pose, Roberto
A1 Gómez Pardo, María Belén
A1 Redondo, María José
A1 Sitjà Bobadilla, Ariadna
A1 Quiroga Berdeal, María Isabel
A1 Martínez Portela, Paulino
K1 RNA-seq
K1 Transcriptome
K1 Turbot
K1 Enteromyxum scophthalmi
K1 Enteromyxosis
K1 Immune response
K1 Apoptosis
K1 Erythropoiesis
K1 Cytoskeleton
K1 Digestive function
AB Background: Enteromyxosis caused by the intestinal myxozoan parasite Enteromyxum scophthalmi is a seriousthreat for turbot (Scophthalmus maximus, L.) aquaculture, causing severe catarrhal enteritis leading to a cachecticsyndrome, with no therapeutic options available. There are still many aspects of host-parasite interaction anddisease pathogenesis that are yet to be elucidated, and to date, no analysis of the transcriptomic changes inducedby E. scophthalmi in turbot organs has been conducted. In this study, RNA-seq technology was applied to headkidney, spleen and pyloric caeca of severely infected turbot with the aim of furthering our understanding of thepathogenetic mechanisms and turbot immune response against enteromyxosis.Results: A huge amount of information was generated with more than 23,000 identified genes in the three organs,amongst which 4,762 were differently expressed (DE) between infected and control fish. Associate gene functionswere studied based on gene ontology terms and available literature, and the most interesting DE genes wereclassified into five categories: 1) immune and defence response; 2) apoptosis and cell proliferation; 3) ironmetabolism and erythropoiesis; 4) cytoskeleton and extracellular matrix and 5) metabolism and digestive function.The analysis of down-regulated genes of the first category revealed evidences of a connexion failure betweeninnate and adaptive immune response, especially represented by a high number of DE interferon-related genes inthe three organs. Furthermore, we found an intense activation of local immune response at intestinal level thatappeared exacerbated, whereas in kidney and spleen genes involved in adaptive immune response were mainlydown-regulated. The apoptotic machinery was only clearly activated in pyloric caeca, while kidney and spleenshowed a marked depression of genes related to erythropoiesis, probably related to disorders in iron homeostasis.The genetic signature of the causes and consequences of cachexia was also demonstrated by the down-regulationof the genes encoding structural proteins and those involved in the digestive metabolism.Conclusions: This transcriptomic study has enabled us to gain a better understanding of the pathogenesis ofenteromyxosis and identify a large number of DE target genes that bring us closer to the development ofstrategies designed to effectively combat this pathogen.
PB BMC
SN 1471-2164
YR 2014
FD 2014
LK http://hdl.handle.net/10347/22078
UL http://hdl.handle.net/10347/22078
LA eng
NO Robledo, D., Ronza, P., Harrison, P. W., Losada, A. P., Bermúdez, R., Pardo, B. G., ... & Martínez, P. (2014). RNA-seq analysis reveals significant transcriptome changes in turbot (Scophthalmus maximus) suffering severe enteromyxosis. Bmc Genomics, 15(1), 1149.
NO This work was funded by the Spanish Ministry of Science and Innovation through the projects AGL2009-13282-C02-01 and −02
DS Minerva
RD 4 may 2026