RT Journal Article
T1 Comparative proteomic profiling of myofibrillar proteins in dry-cured ham with different proteolysis indices and adhesiveness
A1 López-Pedrouso, María
A1 Pérez-Santaescolástica, C.
A1 Franco Ruiz, Daniel
A1 Fulladosa, E.
A1 Carballo, C.
A1 Zapata Babío, José Carlos
A1 Lorenzo, J.M.
K1 Defective ham texture
K1 Instrumental adhesiveness
K1 Meat proteolysis
K1 Meat proteomics
K1 Porcine proteome
K1 Processed meat
AB Excessive proteolysis during dry-cured ham processing may lead to high adhesiveness and consumer dissatisfaction. The aim of this research is to identify biomarkers for proteolysis and adhesiveness. Two hundred biceps femoris porcine muscle samples from Spanish dry-cured ham were firstly evaluated for various physicochemical parameters, including their proteolysis indices and instrumental adhesiveness. Proteins of samples with extreme proteolysis indices were separated by two-dimensional electrophoresis and identified by tandem mass spectrometry (MALDI-TOF/TOF). We found that hams of higher proteolysis index had statistically significant increased adhesiveness. Proteomic analysis revealed statistically significant qualitative and quantitative differences between sample groups. Thus, protein fragments increased remarkably in samples with higher proteolysis index scores. In addition, higher proteolysis index hams showed increased degradation for a total of five non-redundant myofibrillar and sarcoplasmic proteins. However, myosin-1, α-actin and myosin-4 proteins were the biomarkers that underwent the most intense response to proteolysis and adhesiveness.
PB Elsevier
SN 0308-8146
YR 2017
FD 2017-10-16
LK https://hdl.handle.net/10347/39085
UL https://hdl.handle.net/10347/39085
LA eng
NO López-Pedrouso, M., Pérez-Santaescolástica, C., Franco, D., Fulladosa, E., Carballo, J., Zapata, C., & Lorenzo, J. M. (2018). Comparative proteomic profiling of myofibrillar proteins in dry-cured ham with different proteolysis indices and adhesiveness. Food Chemistry, 244, 238-245.
NO This research was supported by Grant RTA 2013-00030-CO3-03 from INIA (Spain).
DS Minerva
RD 27 abr 2026