RT Journal Article
T1 Fast NMR Screening of Macromolecular Complexes by a Paramagnetic Spin Relaxation Filter
A1 Pinto, Luiz F.
A1 Correa, Juan
A1 Zhao, Libo
A1 Riguera Vega, Ricardo
A1 Fernández Megía, Eduardo
AB The paramagnetic spin relaxation filter is described for the rapid NMR screening of intermolecular interactions between ligands and macromolecular anionic receptors with large transverse relaxation enhancements (R2p). The addition of micromolar concentrations of Gd3+ to the mixture produces the immediate broadening/suppression of the NMR signals of interacting species while leaving unaffected those of noncompetitive binders (one-dimensional and two-dimensional experiments). The method is highly sensitive, unveiling interactions that are too weak to generate changes in chemical shifts or relaxation times. It is operationally very simple and hence, it is amenable to ready implementation by nonspecialists. Examples of application such as detecting the formation of interpolymer complexes, cyclodextrin host–guest interactions, and the screening of DNA ligands are included that demonstrate the reliability and broad applicability of the method
PB American Chemical Society
YR 2018
FD 2018-03-12
LK http://hdl.handle.net/10347/16947
UL http://hdl.handle.net/10347/16947
LA eng
NO Pinto, L., Correa, J., Zhao, L., Riguera, R., & Fernandez-Megia, E. (2018). Fast NMR Screening of Macromolecular Complexes by a Paramagnetic Spin Relaxation Filter. ACS Omega, 3, 3, 2974-2983. doi: 10.1021/acsomega.7b02074
NO This work was financially supported by the Spanish Ministry of Economy, Industry, and Competitiveness (MINECO)(CTQ2015-69021-R), the Xunta de Galicia (GRC2014/040 and Centro Singular de Investigacion de Galicia Accreditation 2016-2019, ED431G/09), and the European Union (European Regional Development Fund-ERDF)
DS Minerva
RD 23 abr 2026