RT Journal Article T1 Phylogenetic analysis of the order Pleuronectiformes (Teleostei) based on sequences of 12S and 16S mitochondrial genes A1 Azevedo, Marisa F. C. A1 Oliveira, Claudio A1 Gómez Pardo, María Belén A1 Martínez Portela, Paulino A1 Foresti, Fausto K1 Phylogeny K1 Molecular systematics K1 Mitochondrial DNA K1 Fish evolution K1 Flatfishes AB The fish order Pleuronectiformes, composed of 14 families, has two suborders: Psettodoidei (with one family) andPleuronectoidei (with thirteen families). The relationships among families of Pleuronectoidei and among the generaof their families have extensively been debated and a consensus has not yet been reached. In the present study, partial sequences of the 12S and 16S mitochondrial rRNA genes were obtained from 19 species belonging to the families Achiridae, Bothidae, Cynoglossidae, Paralichthyidae, Pleuronectidae, Scophthalmidae, and Soleidae.Additional sequences of 42 pleuronectiform species were obtained from GenBank. Phylogenetic analyses were conducted by the methods of maximum-parsimony, maximum-likelihood and Bayesian inference. Our results corroborate the monophyletic status of all families, excluding Paralichthyidae. In the family Achiridae, the genusCatathyridium (freshwater) was the sister group of Trinectes (saltwater), and Hypoclinemus (freshwater) was the sister group of Achirus (saltwater). Assuming that the putative ancestor of achirids lived in saltwater, it is suggested thatthe freshwater habitats in South America were colonized independently by different achirid lineages. PB Sociedade Brasileira de Genética SN 1415-4757 YR 2008 FD 2008 LK http://hdl.handle.net/10347/22906 UL http://hdl.handle.net/10347/22906 LA eng NO Azevedo, Marisa F.C., Oliveira, Claudio, Pardo, Belén G., Martínez, Paulino, & Foresti, Fausto. (2008). Phylogenetic analysis of the order Pleuronectiformes (Teleostei) based on sequences of 12S and 16S mitochondrial genes. "Genetics and Molecular Biology", 31(1, Suppl.), 284-292 NO Funds supporting this study were provided by CNPq, CAUNESP and FAPESP, and Universidad de Santiago de Compostela DS Minerva RD 28 abr 2026