RT Journal Article
T1 Synthetic cathinones determination by liquid chromatography-mass spectrometry after ultrasound membrane assisted extraction
A1 Suárez Oubiña, Cristian
A1 Cabarcos Fernández, Pamela
A1 Tabernero Duque, María Jesús
A1 Bermejo Barrera, Pilar
A1 Moreda Piñeiro, Antonio
A1 Bermejo Barrera, Ana María
K1 Synthetic cathinones
K1 Urine
K1 LC-MS/MS
K1 Ultrasounds
K1 Membrane assisted solvent extraction
AB The extraction time for membrane-assisted solvent extraction (MASE) when isolating synthetic cathinones from urine has been speeded up to 30 min by using ultrasounds. Separation and determination of eleven synthetic cathinones was further performed liquid chromatography-tandem mass spectrometry (LC-MS/MS). The ultrasound assisted MASE consisted of adjusting 5.0 mL of urine at pH 11.8 and performing the extractive process with 400 μL of n-hexane as an acceptor phase inside the polypropylene membrane under ultrasonication at room temperature. Synthetic cathinones exhibiting LogP values higher than 2.0, such as 3,4-dimethyl methcathinone, methylendioxypyrovalerone, and naphyrone), were efficiently extracted and pre-concentrated. Other synthetic cathinones (LogP between 2.0 and 0.7) were found to exhibit a lower mass transfer. The method was found to be matrix dependent, and a matrix-matched calibration was required for measurements. The achieved limits of detection (LOD) were between 0.03 (flephedrone) to 0.29 (ethylone) µg L-1, with relative standard deviations (RSDs) within the 6–14% and 7–19% ranges for intraday and inter-day assays, and intraday and inter-day analytical recoveries from 84 to 115% and 85 to 118%, respectively. The developed method was finally applied to urine samples from volunteers attending a music festival
PB Elsevier
YR 2023
FD 2023
LK http://hdl.handle.net/10347/30685
UL http://hdl.handle.net/10347/30685
LA eng
NO Microchemical Journal 191 (2023) 108869
NO This work was supported by the Dirección Xeral de I + D – Xunta de Galicia Grupos de Referencia Competitiva (project number ED431C 2022/029)
DS Minerva
RD 24 abr 2026