RT Journal Article
T1 In Vitro Anti-Biofilm and Antibacterial Properties of Streptococcus downii sp. nov.
A1 Cuenca, Maigualida
A1 Sánchez, María del Carmen
A1 Diz Dios, Pedro
A1 Martínez Lamas, Lucía
A1 Álvarez, Maximiliano
A1 Limeres Posse, Jacobo
A1 Sanz, Mariano
A1 Herrera, David
K1 Oral biofilm
K1 Streptococcus downii sp. nov.
K1 Caries
K1 Streptococcus mutans
K1 Periodontal diseases
K1 Aggregatibacter actinomycetemcomitans
AB The aim of this study was to evaluate the potential anti-biofilm and antibacterial activities of Streptococcus downii sp. nov. To test anti-biofilm properties, Streptococcus mutans, Actinomyces naeslundii, Veillonella parvula, Fusobacterium nucleatum, Porphyromonas gingivalis, and Aggregatibacter actinomycetemcomitans were grown in a biofilm model in the presence or not of S. downii sp. nov. for up to 120 h. For the potential antibacterial activity, 24 h-biofilms were exposed to S. downii sp. nov for 24 and 48 h. Biofilms structures and bacterial viability were studied by microscopy, and the effect in bacterial load by quantitative polymerase chain reaction. A generalized linear model was constructed, and results were considered as statistically significant at p < 0.05. The presence of S. downii sp. nov. during biofilm development did not affect the structure of the community, but an anti-biofilm effect against S. mutans was observed (p < 0.001, after 96 and 120 h). For antibacterial activity, after 24 h of exposure to S. downii sp. nov., counts of S. mutans (p = 0.019) and A. actinomycetemcomitans (p = 0.020) were significantly reduced in well-structured biofilms. Although moderate, anti-biofilm and antibacterial activities of S. downii sp. nov. against oral bacteria, including some periodontal pathogens, were demonstrated in an in vitro biofilm model
PB MDPI
YR 2021
FD 2021
LK http://hdl.handle.net/10347/26215
UL http://hdl.handle.net/10347/26215
LA eng
NO Microorganisms 2021, 9(2), 450; https://doi.org/10.3390/microorganisms9020450
NO This research was co-funded by Xunta de Galicia under Ignicia Programme, Axencia Galega de Innovación, GAIN (12/08/2016; GRANT_NUMBER: IN855A)
DS Minerva
RD 24 abr 2026