RT Journal Article
T1 Effects of eslicarbazepine acetate on acute and chronic latrunculin A-induced seizures and extracellular amino acid levels in the mouse hippocampus
A1 Sierra Paredes, Germán
A1 Loureiro, Ana I.
A1 Wright, Lyndon C.
A1 Sierra Marcuño, Germán
A1 Silva, Patrício Soares da
K1 Anticonvulsant drugs
K1 Eslicarbazepine acetate
K1 Eslicarbazepine
K1 Latrunculin A-induced seizures
K1 Taurine
K1 Glycine
K1 Aspartate
K1 Glutamate
AB Background: Latrunculin A microperfusion of the hippocampus induces acute epileptic seizures and long-termbiochemical changes leading to spontaneous seizures. This study tested the effect of eslicarbazepine acetate (ESL),a novel antiepileptic drug, on latrunculin A-induced acute and chronic seizures, and changes in brain amino acidextracellular levels. Hippocampi of Swiss mice were continuously perfused with a latrunculin A solution (4 μM, 1 μl/min,7 h/day) with continuous EEG and videotape recording for 3 consecutive days. Microdialysate samples were analyzedby HPLC and fluorescence detection of taurine, glycine, aspartate, glutamate and GABA. Thereafter, mice werecontinuously video monitored for two months to identify chronic spontaneous seizures or behavioral changes.Control EEG recordings (8 h) were performed in all animals at least once a week for a minimum of one month.Results: Oral administration of ESL (100 mg/kg), previous to latrunculin A microperfusion, completely preventedacute latrunculin A-induced seizures as well as chronic seizures and all EEG chronic signs of paroxysmal activity.Hippocampal extracellular levels of taurine, glycine and aspartate were significantly increased during latrunculin Amicroperfusion, while GABA and glutamate levels remained unchanged. ESL reversed the increases in extracellulartaurine, glycine and aspartate concentrations to basal levels and significantly reduced glutamate levels. Plasmaand brain bioanalysis showed that ESL was completely metabolized within 1 h after administration to mainlyeslicarbazepine, its major active metabolite.Conclusion: ESL treatment prevented acute latrunculin A-induced seizures as well as chronic seizures and all EEGchronic signs of paroxysmal activity, supporting a possible anti-epileptogenic effect of ESL in mice.
PB BMC
SN 1471-2202
YR 2014
FD 2014
LK http://hdl.handle.net/10347/22082
UL http://hdl.handle.net/10347/22082
LA eng
NO Sierra-Paredes, G., Loureiro, A. I., Wright, L. C., Sierra-Marcuño, G., & Soares-da-Silva, P. (2014). Effects of eslicarbazepine acetate on acute and chronic latrunculin A-induced seizures and extracellular amino acid levels in the mouse hippocampus. BMC neuroscience, 15(1), 134.
NO This study was sponsored by BIAL – Portela & Cª, S.A.
DS Minerva
RD 24 abr 2026