RT Journal Article T1 Characterization of multilayer bacterial cellulose-chitosan films loaded with grape bagasse antioxidant extract: Insights into spectral and water properties, microstructure, and antioxidant activity A1 Cazón Díaz, Patricia A1 Puertas Hernando, Gema Manuela A1 Vázquez Vázquez, Manuel K1 Chitosan K1 Bacterial cellulose K1 Active packaging AB This work explores the development and characterization of composite multilayer films comprising bacterial cellulose (BC) and chitosan enriched with antioxidant compounds from grape bagasse extract (GE) and glycerol. SEM images revealed a compact structure with successful interactions between BC and chitosan, confirmed by FT-IR analysis. Equilibrium moisture content, water vapor permeability (WVP), swollen capacity, and solubility were systematically investigated, unveiling the influence of glycerol and GE concentrations. Moisture content increased with elevated glycerol and GE levels, attributed to their hydrophilic nature. WVP rose with higher concentrations of hydrophilic compounds, affecting the films' permeability. Swollen capacity decreased, and solubility increased with the addition of GE and glycerol, indicating a more compact film structure. The incorporation of GE conferred antioxidant properties to the films, as evidenced by DPPH and ABTS+ assays, and Total Phenolic Content (TPC) determination. TPC values varied from 0 to 1.75 mg GAE/g dried film, depending on GE. Fourier Transform Infrared Spectroscopy (FT-IR) highlighted polymeric associations, and UV–Vis spectra demonstrated enhanced UV-blocking properties. Overall, these multilayer films offer promising applications in food packaging, leveraging natural antioxidant sources for an enhanced functionality PB Elsevier SN 0141-8130 YR 2024 FD 2024 LK http://hdl.handle.net/10347/34768 UL http://hdl.handle.net/10347/34768 LA eng NO International Journal of Biological Macromolecules, Volume 268, Part 2, 2024, 131774 NO he authors appreciate the funding support of Xunta de Galicia, within the postdoctoral fellowship granted to Patricia Cazón Díaz (No. ED481B-2021-040). Authors would like to thank the use of RIAIDT-USC analytical facilities DS Minerva RD 24 abr 2026