RT Journal Article
T1 A chromosome-level genome assembly enables the identification of the follicule stimulating hormone receptor as the master sex-determining gene in the flatfish Solea senegalensis
A1 Herrán Moreno, Roberto de la
A1 Hermida Prieto, Miguel
A1 Rubiolo Gaytán, Juan Andrés
A1 Gómez Garrido, Jèssica
A1 Cruz, Fernando
A1 Robles Rodríguez, Francisca
A1 Navajas Pérez, Rafael
A1 Blanco Hortas, Andrés
A1 Rodríguez Villamayor, Paula
A1 Torres Sabino, Dorinda
A1 Sánchez Quinteiro, Pablo
A1 Ramírez Torres, Daniel
A1 Rodríguez Jiménez, María Esther
A1 Arias Pérez, Alberto
A1 Cross Pacheco, Ismael
A1 Duncan, Neil
A1 Martínez Peña, Teresa
A1 Riaza Cárcamo, Ana
A1 Millán Pérez, Adrián
A1 Rosa, María Cristina de
A1 Pirolli, Davide
A1 Gut, Marta
A1 Bouza Fernández, María Carmen
A1 Robledo, Diego
A1 Rebordinos González, Laureana
A1 Alioto, Tyler
A1 Ruíz Rejón, Carmelo
A1 Martínez Portela, Paulino
AB Sex determination (SD) shows huge variation among fish and a high evolutionary rate, as illustrated by the Pleuronectiformes (flatfishes). This order is characterized by its adaptation to demersal life, compact genomes and diversity of SD mechanisms. Here, we assembled the Solea senegalensis genome, a flatfish of great commercial value, into 82 contigs (614 Mb) combining long- and short-read sequencing, which were next scaffolded using a highly dense genetic map (28,838 markers, 21 linkage groups), representing 98.9% of the assembly. Further, we established the correspondence between the assembly and the 21 chromosomes by using BAC-FISH. Whole genome resequencing of six males and six females enabled the identification of 41 single nucleotide polymorphism variants in the follicle stimulating hormone receptor (fshr) consistent with an XX/XY SD system. The observed sex association was validated in a broader independent sample, providing a novel molecular sexing tool. The fshr gene displayed differential expression between male and female gonads from 86 days post-fertilization, when the gonad is still an undifferentiated primordium, concomitant with the activation of amh and cyp19a1a, testis and ovary marker genes, respectively, in males and females. The Y-linked fshr allele, which included 24 nonsynonymous variants and showed a highly divergent 3D protein structure, was overexpressed in males compared to the X-linked allele at all stages of gonadal differentiation. We hypothesize a mechanism hampering the action of the follicle stimulating hormone driving the undifferentiated gonad toward testis
PB Wiley
SN 1755-098X
YR 2023
FD 2023
LK http://hdl.handle.net/10347/30019
UL http://hdl.handle.net/10347/30019
LA eng
NO de la Herrán, R., Hermida, M., Rubiolo, J. A., Gómez-Garrido, J., Cruz, F., Robles, F., Navajas-Pérez, R., Blanco, A., Villamayor, P. R., Torres, D., Sánchez-Quinteiro, P., Ramirez, D., Rodríguez, M. E., Arias-Pérez, A., Cross, I., Duncan, N., Martínez-Peña, T., Riaza, A., Millán, A. … Martínez, P. (2023). A chromosome-level genome assembly enables the identification of the follicule stimulating hormone receptor as the master sex-determining gene in the flatfish Solea senegalensis. Molecular Ecology Resources, 00, 1– 19. https://doi.org/10.1111/1755-0998.13750
NO European Union's Horizon 2020 research and innovation programme under grant agreement (AQUA-FAANG). Grant Number: 81792. Junta de Andalucía-FEDER Grant. Grant Number: P20-00938. Spanish Ministry of Economy and Competitiveness, FEDER Grants. Grant Numbers: RTI2018-096847-B-C21, RTI2018-096847-B-C22
DS Minerva
RD 24 abr 2026