RT Journal Article
T1 N-acylhomoserine lactone-degrading bacteria isolated from hatchery bivalve larval cultures
A1 Torres, Marta
A1 Romero Bernárdez, Manuel
A1 Prado Plana, Susana
A1 Dubert Pérez, Javier
A1 Tahrioui, Ali
A1 Otero Casal, Ana María
A1 Llamas, Inmaculada
K1 Quorum sensing
K1 Aquaculture
K1 Quorum quenching
K1 Marine bacteria
AB Quorum sensing (QS) systems, which depend on N-acylhomoserine lactone (AHL) signal molecules, mediate the production of virulence factors in many pathogenic microorganisms. One hundred and forty-six bacterial strains, isolated from a bivalve hatchery, were screened for their capacity to degrade five synthetic AHLs [N-butyryl-dl-homoserine lactone (C4-HSL), N-hexanoyl-dl-homoserine lactone (C6-HSL), N-octanoyl-dl-homoserine lactone (C8-HSL), N-decanoyl-dl-homoserine lactone (C10-HSL) and N-dodecanoyl-dl-homoserine lactone (C12-HSL)] using well diffusion agar-plate assays with three biosensors, Chromobacterium violaceum CV026, C. violaceum VIR07 and Agrobacterium tumefaciens NTL4 (pZLR4). The results of these assays led to our choosing four strains (PP2-67, PP2-459, PP2-644 and PP2-663) that were able to degrade all five synthetic AHLs, thus showing a wide spectrum of quorum quenching (QQ) activity. We subsequently confirmed and measured the QQ activity of the four strains by high-performance liquid chromatography plus mass-spectrometry analysis (HPLC–MS). One of the strains which showed the highest AHL-degrading activity, PP2-459, identified as being a member of the genus Thalassomonas was chosen for further study. Finally, using thin-layer chromatography (TLC), we went on to confirm this strain's capacity to degrade the AHLs produced by other non-pathogenic and pathogenic bacteria not taxonomically related.
PB Elsevier
YR 2013
FD 2013-11-07
LK https://hdl.handle.net/10347/41679
UL https://hdl.handle.net/10347/41679
LA eng
NO Microbiological Research Volume 168, Issue 9, 7 November 2013, Pages 547-554
NO This research was supported by grants from the Spanish Ministerio de Educación y Ciencia (CGL2008-02399/BOS; AGL2009-07656) the Consejería de Educación Ciencia y Empresa, of the Andalucian Regional Government (P07-CVI-03150) and the Plan Andaluz de Investigación.
DS Minerva
RD 24 abr 2026